Daniele De Corte (Ocean Technology and Engineering (OTE), National Oceanography Centre Southampton UK) Daniele De Corte Leon Dlugosch (Institute for Chemistry and Biology of the Marine Environment (ICBM), University of Oldenburg Oldenburg Germany) Leon Dlugosch Abhishek Srivastava Abhishek Srivastava Meinhard Simon (Institute for Chemistry and Biology of the Marine Environment (ICBM), University of Oldenburg Oldenburg Germany) Meinhard Simon Dennis A. Hansell (Department of Ocean Sciences University of Miami Miami Florida USA) Dennis A. Hansell Sarah Bercovici (Ocean BioGeosciences (OBG), National Oceanography Centre Southampton UK) Sarah Bercovici Monica Orellana (University of Washington Seattle Washington USA) Monica Orellana Wiley 2025 Biogeochemical cycles in the ocean are strongly influenced by microbial activity, which affects nutrient and organic matter cycling. These processes, influenced by factors such as temperature, salinity, density and inorganic nutrients, drive the vertical stratification of microbial communities, which subsequently influence the chemistry at different depth layers. Sequencing technology has expanded our understanding of oceanic prokaryotic communities' taxonomic and functional potential. However, there is limited information on how these communities vary across gradients. In this study, we conducted metagenomic analyses on samples from the eastern North Pacific, collected across a longitudinal transect around 45°N and throughout the entire water column. We assessed taxonomic and functional classification, focusing on the roles of prokaryotic communities in biogeochemical cycling. Our results revealed that the surface community was dominated by the SAR11 clade, followed by Flavobacterales and Rhodobacterales. The deep layers harboured a more diverse community, where Thaumarchaeota accounted for the most significant proportion. This clear taxonomic stratification led to variations in the communities' functional capabilities across different depth layers. Photosynthesis and heterotrophy dominated the surface layers, whereas the deeper layers exhibited a mix of metabolic features, allowing organisms to potentially utilise both inorganic and organic carbon sources. PDFDocument eng 2026-03-03T11:40:21.638309Z 2025 Diversity Marine Ecoysystem Metabolic Potential Prokaryotes 7294799 b application/pdf http://creativecommons.org/licenses/by/4.0/