https://phaidra.vetmeduni.ac.at/o:4944 A cross section through mosquitoes of Bosnia and Herzegovina: Barcodes, blood meals and pathogens Hoxha, Ina Ina Hoxha Dervović, Jovana Jovana Dervović Unterköfler, Maria Sophia Maria Sophia Unterköfler 0000-0003-2947-1423 University of Veterinary Medicine Vienna Schlamadinger, Lisa Lisa Schlamadinger University of Veterinary Medicine Vienna Situmorang, Tanto Tanto Situmorang University of Veterinary Medicine Vienna Fuehrer, Hans-Peter Hans-Peter Fuehrer 0000-0002-4178-0133 University of Veterinary Medicine Vienna Obwaller, Adelheid G. Adelheid G. Obwaller Sekulin, Karin Karin Sekulin Camp, Jeremy V. Jeremy V. Camp Harl, Josef Josef Harl 0000-0002-4915-3943 University of Veterinary Medicine Vienna Walochnik, Julia Julia Walochnik Alić, Amer Amer Alić Kniha, Edwin Edwin Kniha Elsevier journal article open access eng Mosquitoes are important vectors of human and animal pathogens, yet data on their diversity and vector potential remain scarce for the central Balkan country Bosnia and Herzegovina. This study aimed to assess mosquito species composition, associated pathogens, and the potential public health risks in BIH. Adult mosquitoes were collected with light traps, identified morphologically and by barcoding, and screened molecularly for various pathogens, including West Nile virus (WNV), Dirofilaria spp., Trypanosoma spp., and Plasmodium spp. A total of 2691 mosquitoes of 17 species were identified, with Culex pipiens/torrentium being most abundant and new records of Aedes albopictus and Ae. japonicus japonicus. The first detection of WNV (lineage 2) RNA in mosquitoes in BIH highlights the potential risk of circulation in the region, aligning with findings from neighboring countries. In addition, DNA of filarioid nematodes (Dirofilaria immitis, D. repens, and Setaria tundra) were detected, underscoring their role as potential vectors of zoonotic dirofilariasis. Also, Trypanosoma and Plasmodium DNA was detected, warranting further investigation into the possible involvement of mosquitoes in their transmission. The detection of invasive Aedes species and mosquito-borne pathogens emphasize the need for strengthened vector surveillance in southeastern Europe, particularly in BIH. This study provides the first barcode inventory of 17 mosquito species and novel molecular evidence of mosquito-borne pathogens in BIH, offering valuable baseline data for future epidemiological assessments and sustained entomological surveillance. Aedes Albopictus Aedes Japonicus Barcoding Culex Dirofilaria Haplotyping One Health West Nile Virus http://creativecommons.org/licenses/by/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:4944/download 10.1016/j.onehlt.2025.101246 https://phaidra.vetmeduni.ac.at/o:605 application/pdf One Health issn:2352-7714 VoR One Health 21 5.72 MB 2025