https://phaidra.vetmeduni.ac.at/o:4912 Impact of Blood Metabolic Profile and Ingestive Behaviours Registered with Noseband Sensor on Methane Emission During Transition Period in Dairy Cows Krištolaitytė, Justina Justina Krištolaitytė 0009-0006-0462-1463 Large Animal Clinic, Veterinary Academy, Lithuanian University of Health Sciences, Tilžės Str. 18, LT-47181 Kaunas, Lithuania Šertvytytė, Greta Greta Šertvytytė 0009-0006-4801-3814 Large Animal Clinic, Veterinary Academy, Lithuanian University of Health Sciences, Tilžės Str. 18, LT-47181 Kaunas, Lithuania Lembovičiūtė, Gabija Gabija Lembovičiūtė 0009-0006-6514-391X Large Animal Clinic, Veterinary Academy, Lithuanian University of Health Sciences, Tilžės Str. 18, LT-47181 Kaunas, Lithuania Arlauskaitė, Samanta Samanta Arlauskaitė 0009-0008-8301-0808 Large Animal Clinic, Veterinary Academy, Lithuanian University of Health Sciences, Tilžės Str. 18, LT-47181 Kaunas, Lithuania Girdauskaitė, Akvilė Akvilė Girdauskaitė Large Animal Clinic, Veterinary Academy, Lithuanian University of Health Sciences, Tilžės Str. 18, LT-47181 Kaunas, Lithuania Juškienė, Violeta Violeta Juškienė 0000-0002-3991-3718 Department of Ecology, Animal Science Institute, Lithuanian University of Health Sciences, R. Zebenkos 12, 82317 Baisogala, Lithuania Juška, Remigijus Remigijus Juška Department of Ecology, Animal Science Institute, Lithuanian University of Health Sciences, R. Zebenkos 12, 82317 Baisogala, Lithuania Baumgartner, Walter Walter Baumgartner 0000-0001-7366-7242 Clinical Department for Farm Animals and Food System Science, Clinical Center for Ruminant and Camelid Medicine, University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria Antanaitis, Ramūnas Ramūnas Antanaitis 0000-0002-9993-2224 Large Animal Clinic, Veterinary Academy, Lithuanian University of Health Sciences, Tilžės Str. 18, LT-47181 Kaunas, Lithuania Džermeikaitė, Karina Karina Džermeikaitė 0009-0005-5343-6590 Large Animal Clinic, Veterinary Academy, Lithuanian University of Health Sciences, Tilžės Str. 18, LT-47181 Kaunas, Lithuania Rutkauskas, Arūnas Arūnas Rutkauskas 0000-0001-8931-0475 Large Animal Clinic, Veterinary Academy, Lithuanian University of Health Sciences, Tilžės Str. 18, LT-47181 Kaunas, Lithuania MDPI journal article open access eng This study investigates the relationship between methane emissions and physiological, behavioural, and haematological parameters in dairy cows during the transition period. Methane emissions were monitored alongside variations in rumination, feeding behaviour, and blood markers three weeks before calving, on calving day, and three weeks post-calving. Cows were retrospectively classified into low, medium, and high rumination groups according to their average daily rumination duration to investigate the effects of behavioural influences. During the prepartum period, the methane concentration was moderately positively correlated with drinking time (r = 0.41, p < 0.01) and weakly negatively correlated with chews per minute (r = −0.358, p < 0.05). Significant negative correlations were noted with chloride (r = −0.42, p < 0.01) and glucose levels (r = −0.41, p < 0.01). Following calving, methane emissions showed a positive correlation with haematocrit (r = 0.41, p < 0.01) and a negative correlation with haemoglobin (r = −0.47, p < 0.01). A haematological analysis revealed a notable negative correlation with platelets during calving (r = −0.64, p < 0.05). Individual dry matter intake (DMI) was recorded for each period, showing a significant drop on calving day. This intake fluctuation coincided with a significant rise in methane yield on calving day (p < 0.001). In the low rumination time group, methane was moderately negatively correlated with rumination chews (r = −0.52, p < 0.05), while in the high rumination group, a moderate negative correlation was observed with drinking gulps (r = −0.42, p < 0.05), and a weak negative correlation was observed with bolus events (r = −0.37, p < 0.05). Despite behavioural variations, methane emissions showed no substantial differences among groups with low, medium, and high rumination times, suggesting a minimal direct influence on rumination duration. These findings emphasise the complex interactions between feed intake, metabolism, and methane emissions, underscoring the importance of integrating behavioural and physiological indicators to develop targeted strategies for enteric methane mitigation while providing baseline data from healthy cows that could guide future research on methane emissions in cows undergoing postpartum metabolic disorders. Methane Emission Innovative Technologies Dairy Cows Blood Samples http://creativecommons.org/licenses/by/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:4912/download 10.3390/life15050760 https://phaidra.vetmeduni.ac.at/o:605 application/pdf Life issn:2075-1729 VoR Life 15 5 596.34 kB 2025