phaidra.vetmeduni.ac.at o:4845 DOI 10.1177/03009858251317466 MicroRNAs in serum and tissue can differentiate splenic hemangiosarcoma from other splenic masses in dogs Splenic masses are common in dogs and vary dramatically in their clinical behavior. Clinically, and even with histology, it can be challenging to differentiate between benign and malignant splenic masses. Hemangiosarcoma (HSA), the most common malignancy of the spleen, is a very aggressive tumor with a poor prognosis. We hypothesize that microRNAs (miRNAs) in mass tissue and serum can differentiate between HSA and other splenic masses. Fifty-nine miRNAs were investigated by reverse-transcription followed by real-time quantitative polymerase chain reaction (RT-qPCR) in serum and/or tissue from dogs with HSAs (serum n = 24 and tissue n = 17; postsplenectomy serum n = 11), lymphomas (serum n = 8 and tissue n = 11), nonangiomatous nonlymphomatous sarcomas (serum n = 6 and tissue n = 10), histiocytic sarcomas (tissue n = 4), benign splenic masses (myelolipomas, nodular hyperplasia, and hematomas; total serum n = 21 and total tissue n = 35), and normal dogs (serum n = 14 and tissue n = 7). Numerous miRNAs were differentially expressed in serum and tissue of HSA cases compared to those with other splenic masses or normal spleens. In serum, our 5-miRNA model (miR-135a-5p, miR-10a, miR-450b, miR-152-3p, and miR-126-5p) accurately classified 100% (24/24) of dogs with HSA from normal dogs and those with a benign splenic mass (recall = 1 for HSA). The overall accuracy of the model was 86%. In HSA and benign splenic mass tissues, our 3-miRNA model (miR-126-5p, miR-502-3p, and miR-452-5p) accurately classified 96% of the cases. This study demonstrates the utility of miRNA models in serum and tissue for screening and diagnosis of HSA in dogs. Future studies include the evaluation of prospective and prediagnosis serum samples. eng Diagnosis Dog Hemangiosarcoma microRNAs Serum Splenic Masses Tissue 2026-02-02T15:51:30.653Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Ludwig;Latasha; FN:Latasha Ludwig X-ORCID:https://orcid.org/0009-0009-8328-1031 END:VCARD BEGIN:VCARD VERSION:3.0 N:Degasperi;Brigitte; FN:Brigitte Degasperi X-ORCID:https://orcid.org/0009-0001-0559-3728 END:VCARD BEGIN:VCARD VERSION:3.0 N:Walter;Ingrid; FN:Ingrid Walter X-ORCID:https://orcid.org/0000-0002-8337-562X END:VCARD BEGIN:VCARD VERSION:3.0 N:Stuart;Deirdre; FN:Deirdre Stuart END:VCARD BEGIN:VCARD VERSION:3.0 N:Foster;Robert A.; FN:Robert A. Foster END:VCARD BEGIN:VCARD VERSION:3.0 N:Wood;Robert Darren; FN:Robert Darren Wood END:VCARD BEGIN:VCARD VERSION:3.0 N:Ali;R. Ayesha; FN:R. Ayesha Ali END:VCARD BEGIN:VCARD VERSION:3.0 N:Wood;Geoffrey A.; FN:Geoffrey A. Wood END:VCARD BEGIN:VCARD VERSION:3.0 N:Treleaven;Heather; FN:Heather Treleaven END:VCARD BEGIN:VCARD VERSION:3.0 N:Khachadoorian;Arlene; FN:Arlene Khachadoorian END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by-nc/4.0 application/pdf 1629229 https://phaidra.vetmeduni.ac.at/o:4845 https://phaidra.vetmeduni.ac.at/api/object/o:4845/thumbnail LOMv1.0 discipline