https://phaidra.vetmeduni.ac.at/o:4765 Whole-genome sequence data of the Listeria monocytogenes 64_37 isolated from a trout fillet Slavko, Aleksandra Aleksandra Slavko Skandamis, Panagiotis N. Panagiotis N. Skandamis Kober-Rychli, Kathrin Kathrin Kober-Rychli 0000-0001-6147-577X University of Veterinary Medicine Vienna Papadimitriou, Konstantinos Konstantinos Papadimitriou 0000-0002-9614-6543 Pemaj, Violeta Violeta Pemaj Drosinos, Eleftherios H. Eleftherios H. Drosinos Elsevier journal article open access eng Listeria monocytogenes is a major foodborne pathogen causing listeriosis disease in humans. Fish is a product that can serve as a transmission source. Here we present the whole-genome sequence (WGS) of L. monocytogenes strain 64_37 isolated from a trout fillet. Initial de novo assembly of Illumina raw reads produced 40 contigs of 3,016,736 bp and a GC % content of 37.85 %. Further reference-based assembly resulted in a high-quality genome consisting of one scaffold with a total length 2,957,126 bp and a completeness of 99.99 %. A rep26 -type replicon plasmid was detected. MLST analysis assigned strain 64_37 to serogroup IIa and sequence type (ST) 204 of clonal complex (CC) 204. Functional annotation using the Rapid Annotation using Subsystem Technology (RAST) server revealed 2,949 coding sequences and 41 RNA sequences. Pan-genome analysis identified 88 unique genes in strain 64_37. Investigation of the pathogenic potential of L. monocytogenes 64_37 revealed the presence of multiple virulence genes. Investigation of the presence of antimicrobial resistance (AMR) genes suggested resistance to lincosamide, cationic peptides, fosfomycin and fluoroquinolone. This data provides an overview of pathogenic potential of L. monocytogenes 64_37 and can be used to further shed light on its presence in fish products. Listeria Monocytogenes Trout Fillet Whole-Genome Sequencing Virulence Antimicrobial Resistance http://creativecommons.org/licenses/by/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:4765/download 10.1016/j.dib.2025.112301 https://phaidra.vetmeduni.ac.at/o:605 application/pdf Data in Brief issn:2352-3409 VoR Data in Brief 63 839.12 kB 2025