phaidra.vetmeduni.ac.at o:4721 DOI 10.3390/ani15152215 Evaluating the Impact of Clinical Mastitis on Ovarian Morphometry and the Effectiveness of the Synchronisation Protocol in Dairy Cows Inflammatory processes within the body have been hypothesised to be causative agents of various health complications, including reproductive issues. This study investigates the effects of mastitis on ovarian morphometry and fertility outcomes while also comparing the effectiveness of different synchronisation protocols in affected cows. Healthy multiparous Holstein dairy cows, in their second to fifth lactations and with an average milk yield of approximately 12,000 kg in the preceding lactation, were selected for this study. The average milk yield per lactation was approximately 12,000 kg, with an average daily milk yield of 30 kg. The aim of this study was to determine the impact of mastitis on the reproductive cycle, ovarian morphometry, and function in dairy cows, as well as its effect on synchronisation efficiency. This study focused on both clinically healthy cows and those diagnosed with clinical mastitis postpartum. Three different synchronisation protocols—OvSynch, G7G, and Presynch—were evaluated at 60 days in milk (DIM). A total of 110 cows were included in this study, with 53 being clinically healthy and 57 affected by mastitis. The results indicated that inflammation affects ovarian morphometric parameters such as the area and the number of functional structures. In addition, the G7G protocol was observed to result in higher conception rates in cows suffering from mastitis following the second insemination. This study demonstrated that cows that developed mastitis within the first 30 DIM exhibited higher conception rates after the first insemination compared to those in cows that developed mastitis later in lactation. eng Oestrus Mastitis Synchronisation Protocol 2025-12-23T09:33:16.864Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Lembovičiūtė;Gabija; FN:Gabija Lembovičiūtė X-ORCID:https://orcid.org/0009-0006-6514-391X END:VCARD BEGIN:VCARD VERSION:3.0 N:Žilaitis;Vytuolis; FN:Vytuolis Žilaitis END:VCARD BEGIN:VCARD VERSION:3.0 N:Baumgartner;Walter; FN:Walter Baumgartner X-ORCID:https://orcid.org/0000-0001-7366-7242 END:VCARD BEGIN:VCARD VERSION:3.0 N:Rutkauskas;Arūnas; FN:Arūnas Rutkauskas END:VCARD BEGIN:VCARD VERSION:3.0 N:Šertvytytė;Greta; FN:Greta Šertvytytė X-ORCID:https://orcid.org/0009-0006-4801-3814 END:VCARD BEGIN:VCARD VERSION:3.0 N:Antanaitis;Ramūnas; FN:Ramūnas Antanaitis X-ORCID:https://orcid.org/0000-0002-9993-2224 END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 926167 https://phaidra.vetmeduni.ac.at/o:4721 https://phaidra.vetmeduni.ac.at/api/object/o:4721/thumbnail LOMv1.0 discipline