phaidra.vetmeduni.ac.at o:4554 DOI 10.3390/vaccines13070740 Challenges and Lessons Learned from a Field Trial on the Understanding of the Porcine Respiratory Disease Complex Background/Objectives: The porcine respiratory disease complex (PRDC) is a multifaceted, polymicrobial syndrome resulting from a combination of environmental stressors, primary infections (e.g., PRRSV) and secondary infectious agents (viruses and bacteria). PRDC causes severe lung pathology, leading to reduced performance, increased mortality rates, and higher production costs in the global pig industry. Our goal was to conduct a comprehensive study correlating both the anti-PRRSV immune response and 21 secondary infectious agents with PRDC severity. Methods: To this end, PRRSV-negative weaners were vaccinated with a PRRSV-2 MLV and put into a farm with a history of PRDC. Subsequently, anti-PRRSV cellular and antibody responses were monitored pre-vaccination, at 28 days post vaccination (dpv) and during PRDC outbreak (49 dpv). NanoString was used to quantify 21 pathogens within the bronchoalveolar lavage (BAL) at the time of necropsy (51 dpv). PRRSV-2 was present in 53 out of 55 pigs, and the other five pathogens (PCMV, PPIV, B. bronchiseptica, G. parasuis, and M. hyorhinis) were detected in BAL samples. Results: Although the uncontrolled settings of field trials complicated data interpretation, multivariate correlation analyses highlighted valuable lessons: (i) high weaning weight predicted animal resilience to disease and high weight gains correlated with the control of the PRRSV-2 field strain; (ii) most pigs cleared MLV strain within 7 weeks, and the field PRRSV-2 strain was the most prevalent lung pathogen during PRDC; (iii) all pigs developed a systemic PRRSV IgG antibody response which correlated with IgG and IgA levels in BAL; (iv) the induction of anti-field strain-neutralizing antibodies by MLV PRRSV-2 vaccination was both late and limited; (v) cellular immune responses were variable but included strong systemic IFN-γ production against the PRRSV-2 field strain; (vi) the most detected lung pathogens correlated with PRRSV-2 viremia or lung loads; (vii) within the six detected pathogens, two viruses, PRRSV-2 and PCMV, significantly correlated with the severity of the clinical outcome. Conclusions: While a simple and conclusive answer to the multifaceted nature of PRDC remains elusive, the key lessons derived from this unique study provide a valuable framework for future research on porcine respiratory diseases. eng PRDC PRRSV-2 Immunity NanoString 2025-11-17T10:03:23.082Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Crisci;Elisa; FN:Elisa Crisci END:VCARD BEGIN:VCARD VERSION:3.0 N:Kick;Andrew R.; FN:Andrew R. Kick END:VCARD BEGIN:VCARD VERSION:3.0 N:Cortes;Lizette; FN:Lizette Cortes END:VCARD BEGIN:VCARD VERSION:3.0 N:Byrne;John J.; FN:John J. Byrne END:VCARD BEGIN:VCARD VERSION:3.0 N:Amaral;Amanda F.; FN:Amanda F. Amaral END:VCARD BEGIN:VCARD VERSION:3.0 N:Love;Kim; FN:Kim Love END:VCARD BEGIN:VCARD VERSION:3.0 N:Tong;Hao; FN:Hao Tong END:VCARD BEGIN:VCARD VERSION:3.0 N:Zhang;Jianqiang; FN:Jianqiang Zhang END:VCARD BEGIN:VCARD VERSION:3.0 N:Gauger;Phillip; FN:Phillip Gauger END:VCARD BEGIN:VCARD VERSION:3.0 N:Pittman;Jeremy S.; FN:Jeremy S. Pittman END:VCARD BEGIN:VCARD VERSION:3.0 N:Kaeser;Tobias; FN:Tobias Kaeser X-ORCID:https://orcid.org/0000-0001-8764-7971 END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 1316650 https://phaidra.vetmeduni.ac.at/o:4554 https://phaidra.vetmeduni.ac.at/api/object/o:4554/thumbnail LOMv1.0 discipline