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Sample preparation strategies, based on magnetic beads, filter-aided systems, or in-solution digests, are commonly used for proteomic analysis. Time-intensive methods like filter-aided sample preparation (FASP) have led to the development of new, more time-efficient filter-based strategies like suspension trappings (S-Traps) or magnetic bead-based strategies like SP3. S-Traps have been reported as an alternative proteomic sample preparation method as they allow for high sodium dodecyl sulfate (SDS) concentrations to be present in the sample. In this study, we compare the efficiency of different protocols for FASP, SP3, and S-Trap-based digestion of proteins after extraction from Trichomonas vaginalis. Overall, we found a high number of protein IDs for all tested methods and a high degree of reproducibility within each method type. However, FASP with a 3 kDa cutoff filter unit outperformed the other methods analyzed, referring to the number of protein IDs. This is the first work providing the direct comparison of four different bottom-up proteomic approaches regarding the most efficient proteomic sample preparation protocol for the human parasite T. vaginalis."}]}],"bf:provisionActivity":[{"@type":"bf:Publication","bf:agent":[{"@type":"schema:Organization","schema:name":[{"@value":"American Chemical Society"}]}]}],"dce:rights":[{"@language":"eng","@value":"© 2024 The Authors"}],"dce:subject":[{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Peptide Identification"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Peptides and Proteins"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Proteine Identification"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Proteomics"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Sample Preparation"}]}],"dce:title":[{"@type":"bf:Title","bf:mainTitle":[{"@language":"eng","@value":"A Comparison of Bottom-Up Proteomic Sample Preparation Methods for the Human Parasite Trichomonas vaginalis"}]}],"dcterms:accessRights":[{"@type":"skos:Concept","skos:exactMatch":["https:\/\/pid.phaidra.org\/vocabulary\/QW5R-NG4J"],"skos:prefLabel":[{"@language":"eng","@value":"open access"}]}],"dcterms:issued":["2024"],"dcterms:language":["eng"],"dcterms:type":[{"@type":"skos:Concept","skos:exactMatch":["https:\/\/pid.phaidra.org\/vocabulary\/69ZZ-2KGX"],"skos:prefLabel":[{"@language":"eng","@value":"Text"},{"@language":"deu","@value":"Text"},{"@language":"ita","@value":"Testo"}]}],"ebucore:filename":["mayr-et-al-2024-a-comparison-of-bottom-up-proteomic-sample-preparation-methods-for-the-human-parasite-trichomonas.pdf"],"ebucore:hasMimeType":["application\/pdf"],"edm:hasType":[{"@type":"skos:Concept","skos:exactMatch":["https:\/\/pid.phaidra.org\/vocabulary\/VKA6-9XTY"],"skos:prefLabel":[{"@language":"eng","@value":"journal article"},{"@language":"deu","@value":"Wissenschaftlicher Artikel"},{"@language":"ita","@value":"Articolo di rivista"}]}],"edm:rights":["http:\/\/creativecommons.org\/licenses\/by\/4.0\/"],"oaire:version":[{"@type":"skos:Concept","skos:exactMatch":["https:\/\/pid.phaidra.org\/vocabulary\/PMR8-3C8D"],"skos:prefLabel":[{"@language":"eng","@value":"version of record (published version)"}]}],"rdam:P30004":[{"@type":"ids:doi","@value":"10.1021\/acsomega.3c10040"}],"rdau:P60193":[{"@type":"schema:CreativeWork","bibo:issue":["8"],"bibo:volume":["9"],"dce:title":[{"@type":"bf:Title","bf:mainTitle":[{"@language":"eng","@value":"ACS Omega"}]}],"dcterms:issued":["2024"],"ids:issn":["2470-1343"]}],"role:aut":[{"@type":"schema:Person","schema:affiliation":[{"@type":"schema:Organization","schema:name":[{"@value":"University of Veterinary Medicine Vienna"}],"skos:exactMatch":["https:\/\/ror.org\/01w6qp003"]}],"schema:familyName":[{"@value":"Mayr"}],"schema:givenName":[{"@value":"Anna-Lena"}],"skos:exactMatch":[{"@type":"ids:orcid","@value":"0009-0007-8462-2091"}]},{"@type":"schema:Person","schema:affiliation":[{"@type":"schema:Organization","schema:name":[{"@value":"University of Veterinary Medicine Vienna"}],"skos:exactMatch":["https:\/\/ror.org\/01w6qp003"]}],"schema:familyName":[{"@value":"Hummel"}],"schema:givenName":[{"@value":"Karin"}],"skos:exactMatch":[{"@type":"ids:orcid","@value":"0000-0001-5833-6832"}]},{"@type":"schema:Person","schema:familyName":[{"@value":"Leitsch"}],"schema:givenName":[{"@value":"David"}]},{"@type":"schema:Person","schema:affiliation":[{"@type":"schema:Organization","schema:name":[{"@value":"University of Veterinary Medicine Vienna"}],"skos:exactMatch":["https:\/\/ror.org\/01w6qp003"]}],"schema:familyName":[{"@value":"Razzazi-Fazeli"}],"schema:givenName":[{"@value":"Ebrahim"}],"skos:exactMatch":[{"@type":"ids:orcid","@value":"0000-0002-3343-0301"}]}],"schema:numberOfPages":["10"]}