
<lom:lom xmlns:lom="https://oer-repo.uibk.ac.at/lom" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://oer-repo.uibk.ac.at/lom/latest https://w3id.org/oerbase/profiles/lomuibk/latest/schemas/lom-uibk.xsd">
  
<lom:general>
  
<lom:identifier>
  
<lom:catalog>phaidra.vetmeduni.ac.at</lom:catalog>

  
<lom:entry>
  
<lom:langstring xml:lang="x-none">o:3989</lom:langstring>

  
</lom:entry>

  
</lom:identifier>

  
<lom:identifier>
  
<lom:catalog>DOI</lom:catalog>

  
<lom:entry>
  
<lom:langstring xml:lang="x-none">10.1177/03009858241295399</lom:langstring>

  
</lom:entry>

  
</lom:identifier>

  
<lom:title>
  
<lom:langstring xml:lang="en">Nuclear pleomorphism in canine cutaneous mast cell tumors: Comparison of reproducibility and prognostic relevance between estimates, manual morphometry, and algorithmic morphometry</lom:langstring>

  
</lom:title>

  
<lom:description>
  
<lom:langstring xml:lang="en">Variation in nuclear size and shape is an important criterion of malignancy for many tumor types; however, categorical estimates by pathologists have poor reproducibility. Measurements of nuclear characteristics can improve reproducibility, but current manual methods are time-consuming. The aim of this study was to explore the limitations of estimates and develop alternative morphometric solutions for canine cutaneous mast cell tumors (ccMCTs). We assessed the following nuclear evaluation methods for accuracy, reproducibility, and prognostic utility: (1) anisokaryosis estimates by 11 pathologists; (2) gold standard manual morphometry of at least 100 nuclei; (3) practicable manual morphometry with stratified sampling of 12 nuclei by 9 pathologists; and (4) automated morphometry using deep learning-based segmentation. The study included 96 ccMCTs with available outcome information. Inter-rater reproducibility of anisokaryosis estimates was low (k = 0.226), whereas it was good (intraclass correlation = 0.654) for practicable morphometry of the standard deviation (SD) of nuclear size. As compared with gold standard manual morphometry (area under the ROC curve [AUC] = 0.839, 95% confidence interval [CI] = 0.701-0.977), the prognostic value (tumor-specific survival) of SDs of nuclear area for practicable manual morphometry and automated morphometry were high with an AUC of 0.868 (95% CI = 0.737-0.991) and 0.943 (95% CI = 0.889-0.996), respectively. This study supports the use of manual morphometry with stratified sampling of 12 nuclei and algorithmic morphometry to overcome the poor reproducibility of estimates. Further studies are needed to validate our findings, determine inter-algorithmic reproducibility and algorithmic robustness, and explore tumor heterogeneity of nuclear features in entire tumor sections.</lom:langstring>

  
</lom:description>

  
<lom:language>eng</lom:language>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Animals</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Dogs</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Dog Diseases Pathology</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Dog Diseases Diagnosis</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Prognosis</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Reproducibility of Results</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Cell Nucleus Pathology</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Algorithms</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Skin Neoplasms Veterinary</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Skin Neoplasms Pathology</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Skin Neoplasms Diagnosis</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Male</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Female</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Mastocytosis, Cutaneous Veterinary</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Mastocytosis, Cutaneous Pathology</lom:langstring>

  
</lom:keyword>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Mastocytosis, Cutaneous Diagnosis</lom:langstring>

  
</lom:keyword>

  
</lom:general>

  
<lom:lifecycle>
  
<lom:datetime>2025-03-31T08:19:57.592Z</lom:datetime>

  
<lom:contribute>
  
<lom:role>
  
<lom:source>
  
<lom:langstring xml:lang="x-none">LOMv1.0</lom:langstring>

  
</lom:source>

  
<lom:value>
  
<lom:langstring xml:lang="x-none">Author</lom:langstring>

  
</lom:value>

  
</lom:role>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Haghofer;Andreas;
FN:Andreas Haghofer
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Parlak;Eda;
FN:Eda Parlak
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Bartel;Alexander;
FN:Alexander Bartel
X-ORCID:https://orcid.org/0000-0002-1280-6138
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Donovan;Taryn A.;
FN:Taryn A. Donovan
X-ORCID:https://orcid.org/0000-0001-5740-9550
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Assenmacher;Charles-Antoine;
FN:Charles-Antoine Assenmacher
X-ORCID:https://orcid.org/0000-0003-0073-2730
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Bolfa;Pompei;
FN:Pompei Bolfa
X-ORCID:https://orcid.org/0000-0002-2903-1535
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Dark;Michael J.;
FN:Michael J. Dark
X-ORCID:https://orcid.org/0000-0002-6547-1298
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Fuchs-Baumgartinger;Andrea;
FN:Andrea Fuchs-Baumgartinger
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Klang;Andrea;
FN:Andrea Klang
X-ORCID:https://orcid.org/0000-0001-5352-5757
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Jäger;Kathrin;
FN:Kathrin Jäger
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Klopfleisch;Robert;
FN:Robert Klopfleisch
X-ORCID:https://orcid.org/0000-0002-6308-0568
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Merz;Sophie;
FN:Sophie Merz
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Richter;Barbara;
FN:Barbara Richter
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Schulman;F. Yvonne;
FN:F. Yvonne Schulman
X-ORCID:https://orcid.org/0000-0002-7454-8517
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Janout;Hannah;
FN:Hannah Janout
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Ganz;Jonathan;
FN:Jonathan Ganz
X-ORCID:https://orcid.org/0009-0008-1299-8716
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Scharinger;Josef;
FN:Josef Scharinger
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Aubreville;Marc;
FN:Marc Aubreville
X-ORCID:https://orcid.org/0000-0002-5294-5247
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Winkler;Stephan M.;
FN:Stephan M. Winkler
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Kiupel;Matti;
FN:Matti Kiupel
X-ORCID:https://orcid.org/0000-0003-4429-9529
END:VCARD</lom:vcard>

  
</lom:centity>

  
<lom:centity>
  
<lom:vcard>BEGIN:VCARD
VERSION:3.0
N:Bertram;Christof A.;
FN:Christof A. Bertram
X-ORCID:https://orcid.org/0000-0002-2402-9997
END:VCARD</lom:vcard>

  
</lom:centity>

  
</lom:contribute>

  
</lom:lifecycle>

  
<lom:educational>
  
<lom:learningresourcetype>
  
<lom:source>
  
<lom:langstring xml:lang="x-none">https://w3id.org/kim/hcrt/scheme</lom:langstring>

  
</lom:source>

  
<lom:id>https://w3id.org/kim/hcrt/text</lom:id>

  
<lom:entry>
  
<lom:langstring xml:lang="de">Textdokument</lom:langstring>

  
<lom:langstring xml:lang="en">Text</lom:langstring>

  
</lom:entry>

  
</lom:learningresourcetype>

  
</lom:educational>

  
<lom:rights>
  
<lom:copyrightandotherrestrictions>
  
<lom:source>
  
<lom:langstring xml:lang="x-none">LOMv1.0</lom:langstring>

  
</lom:source>

  
<lom:value>
  
<lom:langstring xml:lang="x-none">yes</lom:langstring>

  
</lom:value>

  
</lom:copyrightandotherrestrictions>

  
<lom:description>
  
<lom:langstring xml:lang="x-t-cc-url">https://creativecommons.org/licenses/by/4.0</lom:langstring>

  
</lom:description>

  
</lom:rights>

  
<lom:technical>
  
<lom:format>application/pdf</lom:format>

  
<lom:size>2890275</lom:size>

  
<lom:location>https://phaidra.vetmeduni.ac.at/o:3989</lom:location>

  
<lom:thumbnail>
  
<lom:url>https://phaidra.vetmeduni.ac.at/api/object/o:3989/thumbnail</lom:url>

  
</lom:thumbnail>

  
</lom:technical>

  
<lom:classification>
  
<lom:purpose>
  
<lom:source>
  
<lom:langstring xml:lang="x-none">LOMv1.0</lom:langstring>

  
</lom:source>

  
<lom:value>
  
<lom:langstring xml:lang="x-none">discipline</lom:langstring>

  
</lom:value>

  
</lom:purpose>

  
</lom:classification>

  
</lom:lom>