phaidra.vetmeduni.ac.at o:3900 DOI 10.1007/s00441-024-03937-z Development of a 3D in vitro model to study corpus luteum of felids based on luteinized cells from antral follicles The study aimed to establish a long-term 3D cell culture model using luteinized follicular cells to investigate the functionality and life cycle of the CL in felids. A mixture of cell types from antral follicles was luteinized in vitro and cultured for up to 23 days. The method, initially applied to the domestic cat, was later extended to Persian and Clouded leopards. Antral follicles were isolated and digested with enzymes; then, the cells were subjected to culture. Experimental subsets were treated with/without 1 µg/mL cloprostenol to validate the cell culture model's suitability for functional studies. In domestic cat samples, microscopic evaluation indicated luteinization, which was confirmed by increased progestagen concentrations and IHC staining for HSD3B and CYP11A1. The gene expression of selected steroidogenic factors (HSD3B1, STAR, CYP11A1) and hormone receptors (LHCGR, PTGFR, PRLR) significantly increased, while CYP17A1 expression decreased. Cloprostenol treatment resulted in reduction of steroidogenic activity, proving its suitability for functional studies. Persian and Clouded leopards' cell cultures exhibited similar patterns in progestagen secretion and gene expression, compared to domestic cats. This model, with its defined luteinization, as well as high and stable progestagen production, allows future investigation of factors regulating CL life cycle and function. eng Animals Female Ovarian Follicle Metabolism Ovarian Follicle Cytology Corpus Luteum Cytology Corpus Luteum Metabolism 2025-03-06T11:04:34.190Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Hryciuk;Michał M.; FN:Michał M. Hryciuk X-ORCID:https://orcid.org/0000-0002-3559-9119 END:VCARD BEGIN:VCARD VERSION:3.0 N:Schröter;Filip; FN:Filip Schröter END:VCARD BEGIN:VCARD VERSION:3.0 N:Claaßen;Svenja; FN:Svenja Claaßen X-ORCID:https://orcid.org/0000-0002-4508-3175 END:VCARD BEGIN:VCARD VERSION:3.0 N:Aurich;Christine; FN:Christine Aurich X-ORCID:https://orcid.org/0000-0001-6077-7362 END:VCARD BEGIN:VCARD VERSION:3.0 N:Wauters;Jella; FN:Jella Wauters END:VCARD BEGIN:VCARD VERSION:3.0 N:Haße;Celina; FN:Celina Haße END:VCARD BEGIN:VCARD VERSION:3.0 N:Braun;Beate C.; FN:Beate C. Braun END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 2476335 https://phaidra.vetmeduni.ac.at/o:3900 https://phaidra.vetmeduni.ac.at/api/object/o:3900/thumbnail LOMv1.0 discipline