phaidra.vetmeduni.ac.at o:3890 DOI 10.3390/horticulturae11010062 Advancing Glycyrrhiza glabra L. Cultivation and Hairy Root Transformation and Elicitation for Future Metabolite Overexpression The production of valuable bioactive compounds in the medicinal plant Glycyrrhiza glabra L. (G. glabra) would benefit from biotechnological approaches for the cultivation and induction of metabolite-producing hairy roots. Germination trials were tested to overcome seed dormancy, achieving high germination rates with sulfuric acid treatment. Hairy root cultures of cotyledons using Rhizobium rhizogenes strain 1724 showed the highest transformation efficiency. A fast-growing line, line S, was subsequently exposed to light treatments (red, blue, and blue and red combined) to evaluate their effects on growth, phenolic content, and Ferric Reducing Antioxidant Power (FRAP). Hairy root cultures grown in blue light and in blue and red light combined had higher growth rates than those grown in red light only or in control conditions (dark). FRAP increased over time under all light treatments, including the control, and those cultures exposed to blue and red light combined had higher FRAP than the control. These findings provide valuable insights into conditions for optimal seed germination and hairy root transformation. Treatment of the line S with different qualities of light induced changes in antioxidant capacity and phenolic content, indicating promise for its use in upregulating secondary metabolite production in G. glabra for future biotechnological applications. eng Antioxidant Activities Hot-Water Secondary Metabolites Spectral Quality Light Germination Licorice Biosynthesis Growth Flavonoids 2025-02-27T11:10:45.072Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Afsharzadeh;Negin; FN:Negin Afsharzadeh X-ORCID:https://orcid.org/0000-0002-5808-0993 END:VCARD BEGIN:VCARD VERSION:3.0 N:Paltram;Renate; FN:Renate Paltram END:VCARD BEGIN:VCARD VERSION:3.0 N:Jungwirth;Arne; FN:Arne Jungwirth X-ORCID:https://orcid.org/0000-0002-2962-4015 END:VCARD BEGIN:VCARD VERSION:3.0 N:Tabrizi;Leila; FN:Leila Tabrizi END:VCARD BEGIN:VCARD VERSION:3.0 N:Nazeri;Vahideh; FN:Vahideh Nazeri END:VCARD BEGIN:VCARD VERSION:3.0 N:Kalantari;Hadi; FN:Hadi Kalantari END:VCARD BEGIN:VCARD VERSION:3.0 N:Halbwirth;Heidi; FN:Heidi Halbwirth END:VCARD BEGIN:VCARD VERSION:3.0 N:Samiei;Leila; FN:Leila Samiei X-ORCID:https://orcid.org/0000-0001-6151-7263 END:VCARD BEGIN:VCARD VERSION:3.0 N:Sheehan;Hester; FN:Hester Sheehan X-ORCID:https://orcid.org/0000-0002-2169-5206 END:VCARD BEGIN:VCARD VERSION:3.0 N:Shokrpour;Majid; FN:Majid Shokrpour X-ORCID:https://orcid.org/0000-0001-9377-554X END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 1408204 https://phaidra.vetmeduni.ac.at/o:3890 https://phaidra.vetmeduni.ac.at/api/object/o:3890/thumbnail LOMv1.0 discipline