{"@context":{"aiiso":"http:\/\/purl.org\/vocab\/aiiso\/schema#","arm":"https:\/\/ld4p.github.io\/arm\/core\/ontology\/0.1\/","bf":"http:\/\/id.loc.gov\/ontologies\/bibframe\/","bib":"https:\/\/bibliotek-o.org\/","bibo":"http:\/\/purl.org\/ontology\/bibo\/","cito":"http:\/\/purl.org\/spar\/cito\/","classSchemes":"http:\/\/id.loc.gov\/vocabulary\/classSchemes","dbo":"http:\/\/dbpedia.org\/ontology\/","dce":"http:\/\/purl.org\/dc\/elements\/1.1\/","dcmitype":"http:\/\/dublincore.org\/documents\/2000\/07\/11\/dcmi-type-vocabulary\/#","dcterms":"http:\/\/purl.org\/dc\/terms\/","ebucore":"http:\/\/www.ebu.ch\/metadata\/ontologies\/ebucore\/ebucore","edm":"http:\/\/www.europeana.eu\/schemas\/edm\/","foaf":"http:\/\/xmlns.com\/foaf\/spec\/#","frap":"http:\/\/purl.org\/cerif\/frapo","identifiers":"http:\/\/id.loc.gov\/vocabulary\/identifiers","ids":"http:\/\/id.loc.gov\/vocabulary\/identifiers\/","opaque":"http:\/\/opaquenamespace.org\/","pcdm":"http:\/\/pcdm.org\/models#","phaidra":"https:\/\/phaidra.org\/ontology\/","rdam":"http:\/\/rdaregistry.info\/Elements\/m\/","rdau":"http:\/\/rdaregistry.info\/Elements\/u\/","rdax":"http:\/\/rdaregistry.info\/Elements\/x\/","rdf":"http:\/\/www.w3.org\/1999\/02\/22-rdf-syntax-ns#","rdfs":"https:\/\/www.w3.org\/TR\/rdf-schema\/","relators":"http:\/\/id.loc.gov\/vocabulary\/relators","role":{"@context":{"aut":{"@container":"@list","@id":"http:\/\/id.loc.gov\/vocabulary\/relators\/aut"}}},"schema":"http:\/\/schema.org\/","skos":"http:\/\/www.w3.org\/2004\/02\/skos\/core#","skosxl":"http:\/\/www.w3.org\/2008\/05\/skos-xl"},"@id":"https:\/\/phaidra.vetmeduni.ac.at\/o:3863","bf:note":[{"@type":"bf:Summary","skos:prefLabel":[{"@language":"eng","@value":"N-glycans with complex core chitobiose modifications are observed in various free-living and parasitic nematodes but are absent in mammals. Using Caenorhabditis elegans as a model, we demonstrated that the core N-acetylglucosamine (GlcNAc) residues are modified by three fucosyltransferases (FUTs), namely FUT-1, FUT-6, and FUT-8. Interestingly, FUT-6 can only fucosylate N-glycans lacking the ?1,6-mannose upper arm, indicating that a specific ?-mannosidase is required to generate substrates for subsequent FUT-6 activity. By analyzing the N-glycomes of aman-3 KOs using offline HPLC-MALDI-TOF MS\/MS, we observed that the absence of aman-3 abolishes ?1,3-fucosylation of the distal GlcNAc of N-glycans, which suggests that AMAN-3 is the relevant mannosidase on whose action FUT-6 depends. Enzymatic characterization of recombinant AMAN-3 and confocal microscopy studies using a knock-in strain (aman-3::eGFP) demonstrated a Golgi localization. In contrast to the classical Golgi ?-mannosidase II (AMAN-2), AMAN-3 displayed a cobalt-dependent ?1,6-mannosidase activity toward N-glycans. Using AMAN-3 and other C. elegans glycoenzymes, we were able to mimic nematode N-glycan biosynthesis in vitro by remodeling a fluorescein conjugated-glycan and generate a tri-fucosylated structure. In addition, using a high-content computer-assisted C. elegans analysis platform, we observed that aman-3 deficient worms display significant developmental\ndelays, morphological, and behavioral alterations in comparison to the WT. Our data demonstrated that AMAN-3 is a Golgi ?-mannosidase required for core fucosylation of the distal GlcNAc of N-glycans. This enzyme is essential for the formation of the unusual tri-fucosylated chitobiose modifications in nematodes, which may play important roles in nematode development and behavior."}]}],"bf:provisionActivity":[{"@type":"bf:Publication","bf:agent":[{"@type":"schema:Organization","schema:name":[{"@value":"Elsevier"}]}]}],"dce:rights":[{"@language":"eng","@value":"Copyright © 2024 The Authors"}],"dce:subject":[{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Animals"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Polysaccharides Metabolism"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Golgi Apparatus Metabolism"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Golgi Apparatus Enzymology"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Alpha-Mannosidase Metabolism"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Alpha-Mannosidase Genetics"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Caenorhabditis Elegans Proteins"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Glycosylation"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Fucose Metabolism"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Fucosyl Transferases"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Fucosyl Metabolism"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Fucosyl Genetics"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Caenorhabditis Elegans Metabolism"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Caenorhabditis Elegans Enzymology"}]},{"@type":"skos:Concept","skos:prefLabel":[{"@language":"eng","@value":"Caenorhabditis Elegans Genetics"}]}],"dce:title":[{"@type":"bf:Title","bf:mainTitle":[{"@language":"eng","@value":"N-glycan Core Tri-fucosylation Requires Golgi ?-mannosidase III Activity that Impacts Nematode Growth and Behaviour"}]}],"dcterms:accessRights":[{"@type":"skos:Concept","skos:exactMatch":["https:\/\/pid.phaidra.org\/vocabulary\/QW5R-NG4J"],"skos:prefLabel":[{"@language":"eng","@value":"open access"}]}],"dcterms:issued":["2024"],"dcterms:language":["eng"],"dcterms:type":[{"@type":"skos:Concept","skos:exactMatch":["https:\/\/pid.phaidra.org\/vocabulary\/69ZZ-2KGX"],"skos:prefLabel":[{"@language":"eng","@value":"Text"},{"@language":"deu","@value":"Text"}]}],"ebucore:filename":["J_107202.pdf"],"ebucore:hasMimeType":["application\/pdf"],"edm:hasType":[{"@type":"skos:Concept","skos:exactMatch":["https:\/\/pid.phaidra.org\/vocabulary\/VKA6-9XTY"],"skos:prefLabel":[{"@language":"eng","@value":"journal article"},{"@language":"deu","@value":"Wissenschaftlicher Artikel"}]}],"edm:rights":["http:\/\/creativecommons.org\/licenses\/by\/4.0\/"],"oaire:version":[{"@type":"skos:Concept","skos:exactMatch":["https:\/\/pid.phaidra.org\/vocabulary\/PMR8-3C8D"],"skos:prefLabel":[{"@language":"eng","@value":"version of record (published version)"}]}],"rdam:P30004":[{"@type":"ids:doi","@value":"10.1016\/j.jbc.2024.107944"}],"rdau:P60193":[{"@type":"schema:CreativeWork","bibo:issue":["12"],"bibo:volume":["300"],"dce:title":[{"@type":"bf:Title","bf:mainTitle":[{"@language":"eng","@value":"Journal of Biological Chemistry"}]}],"dcterms:issued":["2024"],"ids:issn":["1083-351X"]}],"role:aut":[{"@type":"schema:Person","schema:affiliation":[{"@type":"schema:Organization","schema:name":[{"@value":"University of Veterinary Medicine Vienna"}],"skos:exactMatch":["https:\/\/ror.org\/01w6qp003"]}],"schema:familyName":[{"@value":"Kendler"}],"schema:givenName":[{"@value":"Jonatan"}]},{"@type":"schema:Person","schema:familyName":[{"@value":"Wöls"}],"schema:givenName":[{"@value":"Florian"}]},{"@type":"schema:Person","schema:familyName":[{"@value":"Thapliyal"}],"schema:givenName":[{"@value":"Saurabh"}]},{"@type":"schema:Person","schema:familyName":[{"@value":"Arcalis"}],"schema:givenName":[{"@value":"Elsa"}]},{"@type":"schema:Person","schema:affiliation":[{"@type":"schema:Organization","schema:name":[{"@value":"University of Veterinary Medicine Vienna"}],"skos:exactMatch":["https:\/\/ror.org\/01w6qp003"]}],"schema:familyName":[{"@value":"Gabriel"}],"schema:givenName":[{"@value":"Hanna"}]},{"@type":"schema:Person","schema:familyName":[{"@value":"Kubitschek"}],"schema:givenName":[{"@value":"Sascha"}]},{"@type":"schema:Person","schema:familyName":[{"@value":"Malzl"}],"schema:givenName":[{"@value":"Daniel"}]},{"@type":"schema:Person","schema:familyName":[{"@value":"Strobl"}],"schema:givenName":[{"@value":"Maria R."}]},{"@type":"schema:Person","schema:familyName":[{"@value":"Palmberger"}],"schema:givenName":[{"@value":"Dieter"}]},{"@type":"schema:Person","schema:familyName":[{"@value":"Luber"}],"schema:givenName":[{"@value":"Thomas"}]},{"@type":"schema:Person","schema:familyName":[{"@value":"Unverzagt"}],"schema:givenName":[{"@value":"Carlo"}]},{"@type":"schema:Person","schema:familyName":[{"@value":"Paschinger"}],"schema:givenName":[{"@value":"Katharina"}]},{"@type":"schema:Person","schema:familyName":[{"@value":"Glauser"}],"schema:givenName":[{"@value":"Dominique A."}]},{"@type":"schema:Person","schema:familyName":[{"@value":"Wilson"}],"schema:givenName":[{"@value":"Iain B. H."}]},{"@type":"schema:Person","schema:affiliation":[{"@type":"schema:Organization","schema:name":[{"@value":"University of Veterinary Medicine Vienna"}],"skos:exactMatch":["https:\/\/ror.org\/01w6qp003"]}],"schema:familyName":[{"@value":"Yan"}],"schema:givenName":[{"@value":"Shi"}],"skos:exactMatch":[{"@type":"ids:orcid","@value":"0000-0003-4949-9140"}]}],"schema:numberOfPages":["19"]}