phaidra.vetmeduni.ac.at o:3843 DOI 10.1098/rspb.2024.1970 Eco-evolutionary dynamics of host-microbiome interactions in a natural population of closely related mouse subspecies and their hybrids Closely related host species share similar symbionts, but the effects of host genetic admixture and environmental conditions on these communities remain largely unknown. We investigated the influence of host genetic admixture and environmental factors on the intestinal prokaryotic and eukaryotic communities (fungi, parasites) of two house mouse subspecies (Mus musculus domesticus and M. m. musculus) and their hybrids in two settings: (i) wild-caught mice from the European hybrid zone and (ii) wild-derived inbred mice in a controlled laboratory environment before and during a community perturbation (infection). In wild-caught mice, environmental factors strongly predicted the overall microbiome composition. Subspecies' genetic distance significantly influenced the overall microbiome composition, and each component (bacteria, parasites and fungi). While hybridization had a weak effect, it significantly impacted fungal composition. We observed similar patterns in wild-derived mice, where genetic distances and hybridization influenced microbiome composition, with fungi being more stable to infection-induced perturbations than other microbiome components. Subspecies' genetic distance has a stronger and consistent effect across microbiome components than differences in expected heterozygosity among hybrids, suggesting that host divergence and host filtering play a key role in microbiome divergence, influenced by environmental factors. Our findings offer new insights into the eco-evolutionary processes shaping host-microbiome interactions. eng Bacterial Infections Guidelines Aerosol Blood 2025-02-03T15:53:42.837Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Ferreira;Susana C. M.; FN:Susana C. M. Ferreira X-ORCID:https://orcid.org/0000-0003-0265-0210 END:VCARD BEGIN:VCARD VERSION:3.0 N:Jarquín-Díaz;Víctor Hugo; FN:Víctor Hugo Jarquín-Díaz X-ORCID:https://orcid.org/0000-0003-3758-1091 END:VCARD BEGIN:VCARD VERSION:3.0 N:Planillo;Aimara; FN:Aimara Planillo END:VCARD BEGIN:VCARD VERSION:3.0 N:Ďureje;Ľudovít; FN:Ľudovít Ďureje END:VCARD BEGIN:VCARD VERSION:3.0 N:Martincová;Iva; FN:Iva Martincová END:VCARD BEGIN:VCARD VERSION:3.0 N:Kramer-Schadt;Stephanie; FN:Stephanie Kramer-Schadt X-ORCID:https://orcid.org/0000-0002-9269-4446 END:VCARD BEGIN:VCARD VERSION:3.0 N:Forslund-Startceva;Sofia K.; FN:Sofia K. Forslund-Startceva END:VCARD BEGIN:VCARD VERSION:3.0 N:Heitlinger;Emanuel; FN:Emanuel Heitlinger END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 1555005 https://phaidra.vetmeduni.ac.at/o:3843 https://phaidra.vetmeduni.ac.at/api/object/o:3843/thumbnail LOMv1.0 discipline