eng Zachhuber, Lena (University of Vienna / Medical University of Vienna) Kuntner, Claudia (Medical University of Vienna) Filip, Thomas (University of Veterinary Medicine Vienna) Mozayani, Behrang (Medical University of Vienna) Berger, Walter (Medical University of Vienna) Wanek, Thomas (Medical University of Vienna) Helbich, Thomas H. (Medical University of Vienna) Hacker, Marcus (Medical University of Vienna) Stanek, Johann (Medical University of Vienna) Vician, Petra (Medical University of Vienna) Scheiner, Stefan (Medical University of Vienna) Löbsch, Mathilde (Medical University of Vienna) MDPI Cholangiocarcinoma (CCA) is a type of primary liver cancer originating from the biliary tract epithelium, characterized by limited treatment options for advanced cases and low survival rates. This study aimed to establish an orthotopic mouse model for CCA and monitor tumor growth using PET/MR imaging. Murine CCA cells were implanted into the liver lobe of male C57BL/6J mice. The imaging groups included contrast-enhanced (CE) MR, CE-MR with static [18F]FDG-PET, and dynamic [18F]FDG-PET. Tumor volume and FDG uptake were measured weekly over four weeks. Early tumor formation was visible in CE-MR images, with a gradual increase in volume over time. Dynamic FDG-PET revealed an increase in the metabolic glucose rate (MRGlu) over time. Blood analysis showed pathological changes in liver-related parameters. Lung metastases were observed in nearly all animals after four weeks. The study concludes that PET-MR imaging effectively monitors tumor progression in the CCA mouse model, providing insights into CCA development and potential treatment strategies. article Cancers 16(45) (2024) application/pdf Brain Transfer Constants; Graphical Evaluation; Input Function; F-18-Fdg Pet; Liver; Kinetics; Mice CC BY 4.0 International http://creativecommons.org/licenses/by/4.0/ doi:10.3390/cancers16142591 https://phaidra.vetmeduni.ac.at/o:3493 Characterization of a Syngeneic Orthotopic Model of Cholangiocarcinoma by [18F]FDG-PET/MRI 2024