phaidra.vetmeduni.ac.at o:3363 DOI 10.1093/jhered/esad015 A chromosome-scale high-contiguity genome assembly of the cheetah (Acinonyx jubatus) The cheetah (Acinonyx jubatus, SCHREBER 1775) is a large felid and is considered the fastest land animal. Historically, it inhabited open grassland across Africa, the Arabian Peninsula, and southwestern Asia; however, only small and fragmented populations remain today. Here, we present a de novo genome assembly of the cheetah based on PacBio continuous long reads and Hi-C proximity ligation data. The final assembly (VMU_Ajub_asm_v1.0) has a total length of 2.38 Gb, of which 99.7% are anchored into the expected 19 chromosome-scale scaffolds. The contig and scaffold N50 values of 96.8 Mb and 144.4 Mb, respectively, a BUSCO completeness of 95.4% and a k-mer completeness of 98.4%, emphasize the high quality of the assembly. Furthermore, annotation of the assembly identified 23,622 genes and a repeat content of 40.4%. This new highly contiguous and chromosome-scale assembly will greatly benefit conservation and evolutionary genomic analyses and will be a valuable resource, e.g., to gain a detailed understanding of the function and diversity of immune response genes in felids. eng Conservation Genomics, Felidae, Hi-C, PacBio, Proximity-Ligation 2024-08-12T09:37:10.987Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Winter;Sven; FN:Sven Winter X-ORCID:https://orcid.org/0000-0002-1890-0977 END:VCARD BEGIN:VCARD VERSION:3.0 N:Meißner;René; FN:René Meißner X-ORCID:https://orcid.org/0000-0003-2829-1607 END:VCARD BEGIN:VCARD VERSION:3.0 N:Greve;Carola; FN:Carola Greve X-ORCID:https://orcid.org/0000-0003-4993-1378 END:VCARD BEGIN:VCARD VERSION:3.0 N:Ben Hamadou;Alexander; FN:Alexander Ben Hamadou END:VCARD BEGIN:VCARD VERSION:3.0 N:Horin;Petr; FN:Petr Horin X-ORCID:https://orcid.org/0000-0002-5548-4212 END:VCARD BEGIN:VCARD VERSION:3.0 N:Prost;Stefan; FN:Stefan Prost X-ORCID:https://orcid.org/0000-0002-6229-3596 END:VCARD BEGIN:VCARD VERSION:3.0 N:Burger;Pamela A.; FN:Pamela A. Burger X-ORCID:https://orcid.org/0000-0002-6941-0257 END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by-nc/4.0 application/pdf 3812561 https://phaidra.vetmeduni.ac.at/o:3363 https://phaidra.vetmeduni.ac.at/api/object/o:3363/thumbnail LOMv1.0 discipline