Winter, Sven (University of Veterinary Medicine, Vienna) Sven Winter Meißner, René (University of Veterinary Medicine, Vienna) René Meißner Greve, Carola (LOEWE Centre for Translational Biodiversity Genomics) Carola Greve Ben Hamadou, Alexander (LOEWE Centre for Translational Biodiversity Genomics) Alexander Ben Hamadou Horin, Petr (University of Veterinary Sciences Brno) Petr Horin Prost, Stefan (University of Veterinary Medicine Vienna / Natural History Museum Vienna / University of Oulu) Stefan Prost Burger, Pamela A. (University of Veterinary Medicine Vienna) Pamela A. Burger Oxford University Press 2023 The cheetah (Acinonyx jubatus, SCHREBER 1775) is a large felid and is considered the fastest land animal. Historically, it inhabited open grassland across Africa, the Arabian Peninsula, and southwestern Asia; however, only small and fragmented populations remain today. Here, we present a de novo genome assembly of the cheetah based on PacBio continuous long reads and Hi-C proximity ligation data. The final assembly (VMU_Ajub_asm_v1.0) has a total length of 2.38 Gb, of which 99.7% are anchored into the expected 19 chromosome-scale scaffolds. The contig and scaffold N50 values of 96.8 Mb and 144.4 Mb, respectively, a BUSCO completeness of 95.4% and a k-mer completeness of 98.4%, emphasize the high quality of the assembly. Furthermore, annotation of the assembly identified 23,622 genes and a repeat content of 40.4%. This new highly contiguous and chromosome-scale assembly will greatly benefit conservation and evolutionary genomic analyses and will be a valuable resource, e.g., to gain a detailed understanding of the function and diversity of immune response genes in felids. PDFDocument en 2024-08-12T09:33:31.065Z Conservation Genomics, Felidae, Hi-C, PacBio, Proximity-Ligation 3812561 b application/pdf CC BY-NC 4.0 International