phaidra.vetmeduni.ac.at o:3284 DOI 10.1007/s11262-022-01952-2 Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (Nyctalus noctula) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study was to evaluate the distribution and host specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand forty-seven bats representing 21 species from Germany, 464 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific real-time RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 1 of 207 (0.5%; Austria), 3 of 245 (1.2%; Germany) and 3 of 20 (15%; Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within bat populations. eng Animals; Chiroptera; Phylogeny; Orthohantavirus Genetics; Europe; Hantavirus Infections Epidemiology Veterinary; RNA, Viral Genetics 2024-07-25T09:37:42.200Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Dafalla;Maysaa; FN:Maysaa Dafalla END:VCARD BEGIN:VCARD VERSION:3.0 N:Ulrich;Rainer G.; FN:Rainer G. Ulrich END:VCARD BEGIN:VCARD VERSION:3.0 N:Heckel;Gerald; FN:Gerald Heckel END:VCARD BEGIN:VCARD VERSION:3.0 N:Fereidouni;Sasan; FN:Sasan Fereidouni X-ORCID:https://orcid.org/0000-0002-3508-991X END:VCARD BEGIN:VCARD VERSION:3.0 N:Drewes;Stephan; FN:Stephan Drewes END:VCARD BEGIN:VCARD VERSION:3.0 N:Rola;Jerzy; FN:Jerzy Rola END:VCARD BEGIN:VCARD VERSION:3.0 N:Wang;Xuejing; FN:Xuejing Wang END:VCARD BEGIN:VCARD VERSION:3.0 N:Freuling;Conrad Martin; FN:Conrad Martin Freuling END:VCARD BEGIN:VCARD VERSION:3.0 N:Müller;Thomas; FN:Thomas Müller END:VCARD BEGIN:VCARD VERSION:3.0 N:Smreczak;Marcin; FN:Marcin Smreczak END:VCARD BEGIN:VCARD VERSION:3.0 N:Gudrun;Gudrun; FN:Gudrun Gudrun END:VCARD BEGIN:VCARD VERSION:3.0 N:Hoffmann;Bernd; FN:Bernd Hoffmann END:VCARD BEGIN:VCARD VERSION:3.0 N:Jeske;Kathrin; FN:Kathrin Jeske END:VCARD BEGIN:VCARD VERSION:3.0 N:Schlottau;Kore; FN:Kore Schlottau END:VCARD BEGIN:VCARD VERSION:3.0 N:Straková;Petra; FN:Petra Straková END:VCARD BEGIN:VCARD VERSION:3.0 N:Orłowska;Anna; FN:Anna Orłowska END:VCARD BEGIN:VCARD VERSION:3.0 N:Keleş;Sinan Julian; FN:Sinan Julian Keleş END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 1583286 https://phaidra.vetmeduni.ac.at/o:3284 https://phaidra.vetmeduni.ac.at/api/object/o:3284/thumbnail LOMv1.0 discipline