https://phaidra.vetmeduni.ac.at/o:3227 Alteration of the temporal association between courtship audio and visual components affects female sexual response Mitoyen, Clémentine Clémentine Mitoyen University of Vienna Fusani, Leonida Leonida Fusani 0000-0001-8900-796X University of Veterinary Medicine Vienna / University of Vienna Quigley, Cliodhna Cliodhna Quigley 0000-0002-7522-4426 University of Veterinary Medicine Vienna / University of Vienna Canoine, Virginie Virginie Canoine University of Vienna Colombo, Silvia Silvia Colombo University of Vienna Wölfl, Simon Simon Wölfl University of Vienna Wiley text eng Some multimodal signals-that is, occurring in more than one sensory modality-appear to carry additional information which is not present when component signals are presented separately. To understand the function of male ring dove's (Streptopelia risoria) multimodal courtship, we used audiovisual playback of male displays to investigate female response to stimuli differing in their audiovisual timing. From natural courtship recordings, we created a shifted stimulus where audio was shifted relative to video by a fixed value and a jittered stimulus, where each call was moved randomly along the visual channel. We presented 3 groups of females with the same stimulus type, that is, control, shifted, and jittered, for 7 days. We recorded their behavior and assessed pre- and post-test blood estradiol concentration. We found that playback exposure increased estradiol levels, confirming that this technique can be efficiently used to study doves' sexual communication. Additionally, chasing behavior (indicating sexual stimulation) increased over experimental days only in the control condition, suggesting a role of multimodal timing on female response. This stresses the importance of signal configuration in multimodal communication, as additional information is likely to be contained in the temporal association between modalities. Cross-Modal Integration; Multisensory Integration; Reproductive-Behavior; Ring-Dove; Multimodal Courtship; Recorded Songs; Columba-Livia; Dance; Choreography; Stimuli http://creativecommons.org/licenses/by-nc-nd/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:3227/download 10.1111/1749-4877.12670 https://phaidra.vetmeduni.ac.at/o:605 application/pdf Integrative Zoology 18(4), 720-735 (2022) Integrative Zoology 18 4 720 735 879.39 kB 2022