University of Veterinary Medicine Vienna Alteration of the temporal association between courtship audio and visual components affects female sexual response Some multimodal signals-that is, occurring in more than one sensory modality-appear to carry additional information which is not present when component signals are presented separately. To understand the function of male ring dove's (Streptopelia risoria) multimodal courtship, we used audiovisual playback of male displays to investigate female response to stimuli differing in their audiovisual timing. From natural courtship recordings, we created a shifted stimulus where audio was shifted relative to video by a fixed value and a jittered stimulus, where each call was moved randomly along the visual channel. We presented 3 groups of females with the same stimulus type, that is, control, shifted, and jittered, for 7 days. We recorded their behavior and assessed pre- and post-test blood estradiol concentration. We found that playback exposure increased estradiol levels, confirming that this technique can be efficiently used to study doves' sexual communication. Additionally, chasing behavior (indicating sexual stimulation) increased over experimental days only in the control condition, suggesting a role of multimodal timing on female response. This stresses the importance of signal configuration in multimodal communication, as additional information is likely to be contained in the temporal association between modalities. en TEXT article Cross-Modal Integration; Multisensory Integration; Reproductive-Behavior; Ring-Dove; Multimodal Courtship; Recorded Songs; Columba-Livia; Dance; Choreography; Stimuli 2022 2022 Mitoyen, Clémentine (University of Vienna) Fusani, Leonida (University of Veterinary Medicine Vienna / University of Vienna) Quigley, Cliodhna (University of Veterinary Medicine Vienna / University of Vienna) Canoine, Virginie (University of Vienna) Colombo, Silvia (University of Vienna) Wölfl, Simon (University of Vienna) Wiley