o:3204 Colostrum as a source of ESBL-Escherichia coli in feces of newborn calves en The aim of the present study was to determine if colostrum and the equipment for harvesting and feeding colostrum are sources of fecal ESBL/AmpC-producing Escherichia coli (ESBL/AmpC-E. coli) in calves. Therefore, 15 male calves fed with pooled colostrum on a dairy farm and held individually in an experimental barn, the colostrum pool and the equipment for harvesting and feeding colostrum were sampled and analyzed for the occurrence of ESBL/AmpC-E. coli. The ESBL-AmpC-E. coli suspicious isolates were subjected to whole-genome sequence analysis. Forty-three of 45 fecal samples were tested positive for ESBL/AmpC-E. coli. In the colostrum sample and in the milking pot, we also found ESBL/AmpC-E. coli. All 45 E. coli isolates were ESBL-producers, mainly commensal sequence type (ST) 10, but also human-extraintestinal pathogenic E. coli ST131 and ST117 were found. The clonal identity of six fecal isolates with the ESBL-E. coli isolate from the colostrum and of five fecal isolates with the strain from the milking pot demonstrates that the hygiene of colostrum or the colostrum equipment can play a significant role in the spread of ESBL-E. coli. Effective sanitation procedures for colostrum harvesting and feeding equipment are crucial to reduce the ESBL-E. coli shedding of neonatal dairy calves. Animals; Colostrummicrobiology; Cattle; Escherichia coliisolation & purification genetics; Fecesmicrobiology; beta-Lactamasesgeneticsmetabolism; Animals, Newborn; Male; Escherichia coli Infectionsmicrobiologyveterinary; Female; Bacterial Proteins genetics metabolism 1552099 10.3390/ijms23031199 2024-07-08T14:51:33.526Z 44 yes 46 Lisa Bachmann University of Applied Science Neubrandenburg / Research Institute of Farm Animal Biology 0000-0003-0681-4999 Laura Weber University of Applied Science Neubrandenburg person 0000-0002-0050-9478 Wendy Liermann Research Institute of Farm Animal Biology person Harald M. Hammon Research Institute of Farm Animal Biology person Cora Delling University of Leipzig person Franziska Dengler University of Veterinary Medicine Vienna / University of Leipzig person 0000-0002-2178-2270 Katharina Schaufler University of Greifswald / Helmholtz Institute for One Health / Christian-Albrecht University Kiel person Michael Schwabe University of Greifswald person Elias Eger Helmholtz Institute for One Health person Karsten Becker Friedrich Loeffler-Institute person Anne Schütz Friedrich-Loeffler-Institute person Timo Homeier-Bachmann Friedrich-Loeffler-Institute person application/pdf 1290925 https://phaidra.vetmeduni.ac.at/o:3204 no yes 16 70 1552253 Scientific Reports 11 14 1 Nature Portfolio 2024