https://phaidra.vetmeduni.ac.at/o:3204 Colostrum as a source of ESBL-Escherichia coli in feces of newborn calves Bachmann, Lisa Lisa Bachmann 0000-0003-0681-4999 University of Applied Science Neubrandenburg / Research Institute of Farm Animal Biology Homeier-Bachmann, Timo Timo Homeier-Bachmann Friedrich-Loeffler-Institute Schütz, Anne Anne Schütz Friedrich-Loeffler-Institute Becker, Karsten Karsten Becker Friedrich Loeffler-Institute Eger, Elias Elias Eger Helmholtz Institute for One Health Schwabe, Michael Michael Schwabe University of Greifswald Schaufler, Katharina Katharina Schaufler University of Greifswald / Helmholtz Institute for One Health / Christian-Albrecht University Kiel Dengler, Franziska Franziska Dengler 0000-0002-2178-2270 University of Veterinary Medicine Vienna / University of Leipzig Delling, Cora Cora Delling University of Leipzig Hammon, Harald M. Harald M. Hammon Research Institute of Farm Animal Biology Weber, Laura Laura Weber 0000-0002-0050-9478 University of Applied Science Neubrandenburg Liermann, Wendy Wendy Liermann Research Institute of Farm Animal Biology Nature Portfolio text eng The aim of the present study was to determine if colostrum and the equipment for harvesting and feeding colostrum are sources of fecal ESBL/AmpC-producing Escherichia coli (ESBL/AmpC-E. coli) in calves. Therefore, 15 male calves fed with pooled colostrum on a dairy farm and held individually in an experimental barn, the colostrum pool and the equipment for harvesting and feeding colostrum were sampled and analyzed for the occurrence of ESBL/AmpC-E. coli. The ESBL-AmpC-E. coli suspicious isolates were subjected to whole-genome sequence analysis. Forty-three of 45 fecal samples were tested positive for ESBL/AmpC-E. coli. In the colostrum sample and in the milking pot, we also found ESBL/AmpC-E. coli. All 45 E. coli isolates were ESBL-producers, mainly commensal sequence type (ST) 10, but also human-extraintestinal pathogenic E. coli ST131 and ST117 were found. The clonal identity of six fecal isolates with the ESBL-E. coli isolate from the colostrum and of five fecal isolates with the strain from the milking pot demonstrates that the hygiene of colostrum or the colostrum equipment can play a significant role in the spread of ESBL-E. coli. Effective sanitation procedures for colostrum harvesting and feeding equipment are crucial to reduce the ESBL-E. coli shedding of neonatal dairy calves. Animals; Colostrummicrobiology; Cattle; Escherichia coliisolation & purification genetics; Fecesmicrobiology; beta-Lactamasesgeneticsmetabolism; Animals, Newborn; Male; Escherichia coli Infectionsmicrobiologyveterinary; Female; Bacterial Proteins genetics metabolism http://creativecommons.org/licenses/by/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:3204/download 10.3390/ijms23031199 https://phaidra.vetmeduni.ac.at/o:605 application/pdf Scientific Reports 14(1) (2024) Scientific Reports 14 1 1.23 MB 2024