phaidra.vetmeduni.ac.at o:3204 DOI 10.3390/ijms23031199 Colostrum as a source of ESBL-Escherichia coli in feces of newborn calves The aim of the present study was to determine if colostrum and the equipment for harvesting and feeding colostrum are sources of fecal ESBL/AmpC-producing Escherichia coli (ESBL/AmpC-E. coli) in calves. Therefore, 15 male calves fed with pooled colostrum on a dairy farm and held individually in an experimental barn, the colostrum pool and the equipment for harvesting and feeding colostrum were sampled and analyzed for the occurrence of ESBL/AmpC-E. coli. The ESBL-AmpC-E. coli suspicious isolates were subjected to whole-genome sequence analysis. Forty-three of 45 fecal samples were tested positive for ESBL/AmpC-E. coli. In the colostrum sample and in the milking pot, we also found ESBL/AmpC-E. coli. All 45 E. coli isolates were ESBL-producers, mainly commensal sequence type (ST) 10, but also human-extraintestinal pathogenic E. coli ST131 and ST117 were found. The clonal identity of six fecal isolates with the ESBL-E. coli isolate from the colostrum and of five fecal isolates with the strain from the milking pot demonstrates that the hygiene of colostrum or the colostrum equipment can play a significant role in the spread of ESBL-E. coli. Effective sanitation procedures for colostrum harvesting and feeding equipment are crucial to reduce the ESBL-E. coli shedding of neonatal dairy calves. eng Animals; Colostrummicrobiology; Cattle; Escherichia coliisolation & purification genetics; Fecesmicrobiology; beta-Lactamasesgeneticsmetabolism; Animals, Newborn; Male; Escherichia coli Infectionsmicrobiologyveterinary; Female; Bacterial Proteins genetics metabolism 2024-07-08T14:57:04.556Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Bachmann;Lisa; FN:Lisa Bachmann X-ORCID:https://orcid.org/0000-0003-0681-4999 END:VCARD BEGIN:VCARD VERSION:3.0 N:Homeier-Bachmann;Timo; FN:Timo Homeier-Bachmann END:VCARD BEGIN:VCARD VERSION:3.0 N:Schütz;Anne; FN:Anne Schütz END:VCARD BEGIN:VCARD VERSION:3.0 N:Becker;Karsten; FN:Karsten Becker END:VCARD BEGIN:VCARD VERSION:3.0 N:Eger;Elias; FN:Elias Eger END:VCARD BEGIN:VCARD VERSION:3.0 N:Schwabe;Michael; FN:Michael Schwabe END:VCARD BEGIN:VCARD VERSION:3.0 N:Schaufler;Katharina; FN:Katharina Schaufler END:VCARD BEGIN:VCARD VERSION:3.0 N:Dengler;Franziska; FN:Franziska Dengler X-ORCID:https://orcid.org/0000-0002-2178-2270 END:VCARD BEGIN:VCARD VERSION:3.0 N:Delling;Cora; FN:Cora Delling END:VCARD BEGIN:VCARD VERSION:3.0 N:Hammon;Harald M.; FN:Harald M. Hammon END:VCARD BEGIN:VCARD VERSION:3.0 N:Weber;Laura; FN:Laura Weber X-ORCID:https://orcid.org/0000-0002-0050-9478 END:VCARD BEGIN:VCARD VERSION:3.0 N:Liermann;Wendy; FN:Wendy Liermann END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 1290925 https://phaidra.vetmeduni.ac.at/o:3204 https://phaidra.vetmeduni.ac.at/api/object/o:3204/thumbnail LOMv1.0 discipline