Bartsch, Silvester J. (Medical University of Vienna) Silvester J. Bartsch Pinker, Katja (Memorial Sloan Kettering Cancer Center) Katja Pinker Helbich, Thomas H. (Medical University of Vienna) Thomas H. Helbich Laimer-Gruber, Daniela (Medical University of Vienna) Daniela Laimer-Gruber Kenner, Lukas (University of Veterinary Medicine Vienna / Medical University of Vienna) Lukas Kenner Fürböck, Christoph (Medical University of Vienna) Christoph Fürböck Friske, Joachim (Medical University of Vienna) Joachim Friske Brožová, Klára (University of Veterinary Medicine Vienna / Medical University of Vienna) Klára Brožová Ehret, Viktoria (Medical University of Vienna) Viktoria Ehret MDPI 2024 Tumor neoangiogenesis is an important hallmark of cancer progression, triggered by alternating selective pressures from the hypoxic tumor microenvironment. Non-invasive, non-contrast-enhanced multiparametric MRI combining blood-oxygen-level-dependent (BOLD) MRI, which depicts blood oxygen saturation, and intravoxel-incoherent-motion (IVIM) MRI, which captures intravascular and extravascular diffusion, can provide insights into tumor oxygenation and neovascularization simultaneously. Our objective was to identify imaging markers that can predict hypoxia-induced angiogenesis and to validate our findings using multiplexed immunohistochemical analyses. We present an in vivo study involving 36 female athymic nude mice inoculated with luminal A, Her2+, and triple-negative breast cancer cells. We used a high-field 9.4-tesla MRI system for imaging and subsequently analyzed the tumors using multiplex immunohistochemistry for CD-31, PDGFR-β, and Hif1-α. We found that the hyperoxic-BOLD-MRI-derived parameter ΔR2* discriminated luminal A from Her2+ and triple-negative breast cancers, while the IVIM-derived parameter fIVIM discriminated luminal A and Her2+ from triple-negative breast cancers. A comprehensive analysis using principal-component analysis of both multiparametric MRI- and mpIHC-derived data highlighted the differences between triple-negative and luminal A breast cancers. We conclude that multiparametric MRI combining hyperoxic BOLD MRI and IVIM MRI, without the need for contrast agents, offers promising non-invasive markers for evaluating hypoxia-induced angiogenesis. PDFDocument en 2024-06-06T09:33:55.711Z Angiogenesis; Hallmarks; Diffusion; Expression; Pericytes; Receptor; Benign; Hif-1 709340 b application/pdf CC BY 4.0 International