phaidra.vetmeduni.ac.at o:3070 DOI 10.3390/ph17010064 Eugenol-Rich Essential Oil from Pimenta dioica: In Vitro and In Vivo Potentialities against Leishmania amazonensis Pimenta dioica L. is one the most recognized species with diverse biological activities. In this study, in vitro activity and in vivo efficacy of essential oil from P. dioica (EO-Pd) was evaluated. The main compound was also included in the animal studies and its in silico prediction related to biological activities, molecular ligands, drug likeness, and ADME (absorption, distribution, metabolism, and excretion) properties are listed. The chemical composition analyzed by GC-MS retrieved 45 components, which the most abundant compound was the eugenol (80.1%). The EO-Pd was able to inhibit the growth of L. amazonensis (IC50 = 9.7 ± 0.7 and 11.3 ± 2.1 µg/mL, promastigotes and amastigotes, respectively). The cytotoxicity assay showed a CC50 of 104.5 ± 0.9 µg/mL and a selectivity index of 9. In the model of cutaneous leishmaniasis in BALB/c mice, the effect of EO-Pd and eugenol was observed after treatment at 30 mg/kg by intralesional route with 5 administrations every 4 days. In the in silico predictions, some targets that justified the antileishmanial activity of eugenol and good drug like properties for this compound, were obtained. This study showed for first time the potential of EO-Pd to inhibit L. amazonensis, which could be linked to the activity of major compound eugenol. eng Antileishmanial Activity; Components; Leaves 2024-06-05T07:44:30.867Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Monzote;Lianet; FN:Lianet Monzote X-ORCID:https://orcid.org/0000-0002-1958-809X END:VCARD BEGIN:VCARD VERSION:3.0 N:Setzer;William N.; FN:William N. Setzer X-ORCID:https://orcid.org/0000-0002-3639-0528 END:VCARD BEGIN:VCARD VERSION:3.0 N:Gille;Lars; FN:Lars Gille X-ORCID:https://orcid.org/0000-0003-1223-0201 END:VCARD BEGIN:VCARD VERSION:3.0 N:Satyal;Prabodh; FN:Prabodh Satyal END:VCARD BEGIN:VCARD VERSION:3.0 N:Gutiérrez;Yamilet I.; FN:Yamilet I. Gutiérrez END:VCARD BEGIN:VCARD VERSION:3.0 N:Scull;Ramón; FN:Ramón Scull END:VCARD BEGIN:VCARD VERSION:3.0 N:Machín;Laura; FN:Laura Machín X-ORCID:https://orcid.org/0000-0002-0708-6789 END:VCARD BEGIN:VCARD VERSION:3.0 N:González;Adiel; FN:Adiel González END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 569277 https://phaidra.vetmeduni.ac.at/o:3070 https://phaidra.vetmeduni.ac.at/api/object/o:3070/thumbnail LOMv1.0 discipline