https://phaidra.vetmeduni.ac.at/o:3048 Polarity reversal of canine intestinal organoids reduces proliferation and increases cell death Csukovich, Georg Georg Csukovich 0000-0001-5615-666X University of Veterinary Medicine Vienna Burgener, Iwan Anton Iwan Anton Burgener 0000-0002-8516-0040 University of Veterinary Medicine Vienna Pratscher, Barbara Barbara Pratscher 0000-0001-5291-1365 University of Veterinary Medicine Vienna Steinborn, Ralf Ralf Steinborn 0000-0001-9287-5039 University of Veterinary Medicine Vienna Tschulenk, Waltraud Waltraud Tschulenk University of Veterinary Medicine Vienna Burger, Stefanie Stefanie Burger University of Veterinary Medicine Vienna Wagner, Maximilian Maximilian Wagner University of Veterinary Medicine Vienna Walter, Ingrid Ingrid Walter 0000-0002-5038-188X University of Veterinary Medicine Vienna Wiley text eng Apical-out intestinal organoids are a relatively simple method of gaining access to the apical cell surface and have faced increasing scientific interest over the last few years. Apical-out organoids can thus be used for disease modelling to compare differing effects on the basolateral versus the apical cell surface. However, these 'inside-out' organoids die relatively quickly and cannot be propagated as long as their basal-out counterparts. Here, we show that apical-out organoids have drastically reduced proliferative potential, as evidenced by immunohistochemical staining and the incorporation of the thymidine analogue EdU. At the same time, cell death levels are increased. Nevertheless, these phenomena cannot be explained by an induction of differentiation, as the gene expression of key marker genes for various cell types does not change over time. Stem-Cells; In-Vitro; Culture http://creativecommons.org/licenses/by/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:3048/download 10.1111/cpr.13544 https://phaidra.vetmeduni.ac.at/o:605 application/pdf Cell Proliferation 57(2) (2023) Cell Proliferation 57 2 947.20 kB 2023