phaidra.vetmeduni.ac.at o:3048 DOI 10.1111/cpr.13544 Polarity reversal of canine intestinal organoids reduces proliferation and increases cell death Apical-out intestinal organoids are a relatively simple method of gaining access to the apical cell surface and have faced increasing scientific interest over the last few years. Apical-out organoids can thus be used for disease modelling to compare differing effects on the basolateral versus the apical cell surface. However, these 'inside-out' organoids die relatively quickly and cannot be propagated as long as their basal-out counterparts. Here, we show that apical-out organoids have drastically reduced proliferative potential, as evidenced by immunohistochemical staining and the incorporation of the thymidine analogue EdU. At the same time, cell death levels are increased. Nevertheless, these phenomena cannot be explained by an induction of differentiation, as the gene expression of key marker genes for various cell types does not change over time. eng Stem-Cells; In-Vitro; Culture 2024-05-29T11:07:35.071Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Csukovich;Georg; FN:Georg Csukovich X-ORCID:https://orcid.org/0000-0001-5615-666X END:VCARD BEGIN:VCARD VERSION:3.0 N:Burgener;Iwan Anton; FN:Iwan Anton Burgener X-ORCID:https://orcid.org/0000-0002-8516-0040 END:VCARD BEGIN:VCARD VERSION:3.0 N:Pratscher;Barbara; FN:Barbara Pratscher X-ORCID:https://orcid.org/0000-0001-5291-1365 END:VCARD BEGIN:VCARD VERSION:3.0 N:Steinborn;Ralf; FN:Ralf Steinborn X-ORCID:https://orcid.org/0000-0001-9287-5039 END:VCARD BEGIN:VCARD VERSION:3.0 N:Tschulenk;Waltraud; FN:Waltraud Tschulenk END:VCARD BEGIN:VCARD VERSION:3.0 N:Burger;Stefanie; FN:Stefanie Burger END:VCARD BEGIN:VCARD VERSION:3.0 N:Wagner;Maximilian; FN:Maximilian Wagner END:VCARD BEGIN:VCARD VERSION:3.0 N:Walter;Ingrid; FN:Ingrid Walter X-ORCID:https://orcid.org/0000-0002-5038-188X END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 969928 https://phaidra.vetmeduni.ac.at/o:3048 https://phaidra.vetmeduni.ac.at/api/object/o:3048/thumbnail LOMv1.0 discipline