https://phaidra.vetmeduni.ac.at/o:2961 Comparative analysis of swine leukocyte antigen gene diversity in Göttingen Minipigs Hammer, Sabine E. Sabine E. Hammer 0000-0003-1475-3938 University of Veterinary Medicine Vienna Figueiredo, Constanca Constanca Figueiredo Hannover Medical School Wenzel, Nadine Nadine Wenzel Hannover Medical School Saalmüller, Armin Armin Saalmüller 0000-0002-7703-3252 University of Veterinary Medicine Vienna Jensen, Andres Eskjær Andres Eskjær Jensen Ellegaard Göttingen Minipigs A/S Stadler, Maria Maria Stadler University of Veterinary Medicine Vienna Sprung, Julia Julia Sprung University of Veterinary Medicine Vienna Kasper, Lena Lena Kasper Merck Healthcare KGaA Hacker, Karolin Karolin Hacker Hannover Medical School Pernold, Clara P. S. Clara P. S. Pernold University of Veterinary Medicine Vienna Groiss, Sandra Sandra Groiss University of Veterinary Medicine Vienna Duckova, Tereza Tereza Duckova University of Veterinary Medicine Vienna Gociman, Monica Monica Gociman University of Veterinary Medicine Vienna Frontiers Media Sa text eng Worldwide, pigs represent economically important farm animals, also representing a preferred preclinical large animal model for biomedical studies. The need for swine leukocyte antigen (SLA) typing is increasing with the expanded use of pigs in translational research, infection studies, and for veterinary vaccine design. Göttingen Minipigs (GMP) attract increasing attention as valuable model for pharmacological studies and transplantation research. This study represents a first-time assessment of the SLA gene diversity in Göttingen Minipigs in combination with a comparative metadata analysis with commercial pig lines. As Göttingen Minipigs could harbor private as well as potential novel SLA allele combinations, future research projects would benefit from the characterization of their SLA background. In 209 Göttingen Minipigs, SLA class I (SLA-1, SLA-2, SLA-3) and class II (DRB1, DQB1, DQA) genes were characterized by PCR-based low-resolution (Lr) haplotyping. Criteria and nomenclature used for SLA haplotyping were proposed by the ISAG/IUIS-VIC SLA Nomenclature Committee. Haplotypes were assigned based on the comparison with already known breed or farm-specific allele group combinations. In total, 14 SLA class I and five SLA class II haplotypes were identified in the studied cohort, to manifest in 26 SLA class I but only seven SLA class II genotypes. The most common SLA class I haplotypes Lr-24.0 (SLA-1*15XX or Blank-SLA-3*04:04-SLA-2*06:01~02) and Lr-GMP-3.0 (SLA-1*16:02-SLA-3*03:04-SLA-2*17:01) occurred at frequencies of 23.44 and 18.66%, respectively. For SLA class II, the most prevalent haplotypes Lr-0.21 (DRB1*01XX-DQB1*05XX-DQA*04XX) and Lr-0.03 (DRB1*03:02-DQB1*03:01-DQA*01XX) occurred at frequencies of 38.28 and 30.38%. The comparative metadata analysis revealed that Göttingen Minipigs only share six SLA class I and two SLA class II haplotypes with commercial pig lines. More importantly, despite the limited number of SLA class I haplotypes, the high genotype diversity being observed necessitates pre-experimental SLA background assessment of Göttingen Minipigs in regenerative medicine, allo-transplantation, and xenograft research. Swine; Humans; Animals; Swine, Miniaturegenetics; Histocompatibility Antigens Class IIgenetics; Histocompatibility Antigens Class Igenetics; Haplotypes http://creativecommons.org/licenses/by/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:2961/download 10.3389/fimmu.2024.1360022 https://phaidra.vetmeduni.ac.at/o:605 application/pdf Frontiers in Microbiology 15 (2024) Frontiers in Microbiology 15 4.00 MB 2024