phaidra.vetmeduni.ac.at o:2961 DOI 10.3389/fimmu.2024.1360022 Comparative analysis of swine leukocyte antigen gene diversity in Göttingen Minipigs Worldwide, pigs represent economically important farm animals, also representing a preferred preclinical large animal model for biomedical studies. The need for swine leukocyte antigen (SLA) typing is increasing with the expanded use of pigs in translational research, infection studies, and for veterinary vaccine design. Göttingen Minipigs (GMP) attract increasing attention as valuable model for pharmacological studies and transplantation research. This study represents a first-time assessment of the SLA gene diversity in Göttingen Minipigs in combination with a comparative metadata analysis with commercial pig lines. As Göttingen Minipigs could harbor private as well as potential novel SLA allele combinations, future research projects would benefit from the characterization of their SLA background. In 209 Göttingen Minipigs, SLA class I (SLA-1, SLA-2, SLA-3) and class II (DRB1, DQB1, DQA) genes were characterized by PCR-based low-resolution (Lr) haplotyping. Criteria and nomenclature used for SLA haplotyping were proposed by the ISAG/IUIS-VIC SLA Nomenclature Committee. Haplotypes were assigned based on the comparison with already known breed or farm-specific allele group combinations. In total, 14 SLA class I and five SLA class II haplotypes were identified in the studied cohort, to manifest in 26 SLA class I but only seven SLA class II genotypes. The most common SLA class I haplotypes Lr-24.0 (SLA-1*15XX or Blank-SLA-3*04:04-SLA-2*06:01~02) and Lr-GMP-3.0 (SLA-1*16:02-SLA-3*03:04-SLA-2*17:01) occurred at frequencies of 23.44 and 18.66%, respectively. For SLA class II, the most prevalent haplotypes Lr-0.21 (DRB1*01XX-DQB1*05XX-DQA*04XX) and Lr-0.03 (DRB1*03:02-DQB1*03:01-DQA*01XX) occurred at frequencies of 38.28 and 30.38%. The comparative metadata analysis revealed that Göttingen Minipigs only share six SLA class I and two SLA class II haplotypes with commercial pig lines. More importantly, despite the limited number of SLA class I haplotypes, the high genotype diversity being observed necessitates pre-experimental SLA background assessment of Göttingen Minipigs in regenerative medicine, allo-transplantation, and xenograft research. eng Swine; Humans; Animals; Swine, Miniaturegenetics; Histocompatibility Antigens Class IIgenetics; Histocompatibility Antigens Class Igenetics; Haplotypes 2024-05-08T08:23:12.252Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Hammer;Sabine E.; FN:Sabine E. Hammer X-ORCID:https://orcid.org/0000-0003-1475-3938 END:VCARD BEGIN:VCARD VERSION:3.0 N:Figueiredo;Constanca; FN:Constanca Figueiredo END:VCARD BEGIN:VCARD VERSION:3.0 N:Wenzel;Nadine; FN:Nadine Wenzel END:VCARD BEGIN:VCARD VERSION:3.0 N:Saalmüller;Armin; FN:Armin Saalmüller X-ORCID:https://orcid.org/0000-0002-7703-3252 END:VCARD BEGIN:VCARD VERSION:3.0 N:Jensen;Andres Eskjær; FN:Andres Eskjær Jensen END:VCARD BEGIN:VCARD VERSION:3.0 N:Stadler;Maria; FN:Maria Stadler END:VCARD BEGIN:VCARD VERSION:3.0 N:Sprung;Julia; FN:Julia Sprung END:VCARD BEGIN:VCARD VERSION:3.0 N:Kasper;Lena; FN:Lena Kasper END:VCARD BEGIN:VCARD VERSION:3.0 N:Hacker;Karolin; FN:Karolin Hacker END:VCARD BEGIN:VCARD VERSION:3.0 N:Pernold;Clara P. S.; FN:Clara P. S. Pernold END:VCARD BEGIN:VCARD VERSION:3.0 N:Groiss;Sandra; FN:Sandra Groiss END:VCARD BEGIN:VCARD VERSION:3.0 N:Duckova;Tereza; FN:Tereza Duckova END:VCARD BEGIN:VCARD VERSION:3.0 N:Gociman;Monica; FN:Monica Gociman END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 4191189 https://phaidra.vetmeduni.ac.at/o:2961 https://phaidra.vetmeduni.ac.at/api/object/o:2961/thumbnail LOMv1.0 discipline