
<resource xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:datacite="http://datacite.org/schema/kernel-4" xmlns="http://namespace.openaire.eu/schema/oaire/" xsi:schemaLocation="http://namespace.openaire.eu/schema/oaire/ https://www.openaire.eu/schema/repo-lit/4.0/openaire.xsd">
  
<datacite:identifier identifierType="URL">https://phaidra.vetmeduni.ac.at/o:2940</datacite:identifier>

  
<datacite:titles>
  
<datacite:title xml:lang="en">Fusarium sporotrichioides Produces Two HT-2-α-Glucosides on Rice</datacite:title>

  
</datacite:titles>

  
<datacite:creators>
  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Svoboda, Thomas</datacite:creatorName>

  
<datacite:givenName>Thomas</datacite:givenName>

  
<datacite:familyName>Svoboda</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0003-0502-301X</datacite:nameIdentifier>

  
<datacite:affiliation>University of Natural Resources and Life Sciences Vienna</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Adam, Gerhard</datacite:creatorName>

  
<datacite:givenName>Gerhard</datacite:givenName>

  
<datacite:familyName>Adam</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0002-3008-3797</datacite:nameIdentifier>

  
<datacite:affiliation>University of Natural Resources and Life Sciences Vienna</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Berthiller, Franz</datacite:creatorName>

  
<datacite:givenName>Franz</datacite:givenName>

  
<datacite:familyName>Berthiller</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0001-5883-2879</datacite:nameIdentifier>

  
<datacite:affiliation>University of Natural Resources and Life Sciences Vienna</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Bacher, Markus</datacite:creatorName>

  
<datacite:givenName>Markus</datacite:givenName>

  
<datacite:familyName>Bacher</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0002-4307-4849</datacite:nameIdentifier>

  
<datacite:affiliation>University of Natural Resources and Life Sciences Vienna / University of Veterinary Medicine Vienna</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Krska, Rudolf</datacite:creatorName>

  
<datacite:givenName>Rudolf</datacite:givenName>

  
<datacite:familyName>Krska</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0001-6843-9755</datacite:nameIdentifier>

  
<datacite:affiliation>University of Natural Resources and Life Sciences Vienna / Austrian Competence Centre for Feed and Food Quality, Safety and Innovation</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Labuda, Roman</datacite:creatorName>

  
<datacite:givenName>Roman</datacite:givenName>

  
<datacite:familyName>Labuda</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0003-0761-178X</datacite:nameIdentifier>

  
<datacite:affiliation>University of Veterinary Medicine Vienna / Research Platform Bioactive Microbial Metabolites</datacite:affiliation>

  
</datacite:creator>

  
<datacite:creator>
  
<datacite:creatorName nameType="Personal">Sulyok, Michael</datacite:creatorName>

  
<datacite:givenName>Michael</datacite:givenName>

  
<datacite:familyName>Sulyok</datacite:familyName>

  
<datacite:nameIdentifier nameIdentifierScheme="ORCID" schemeURI="https://orcid.org/">0000-0002-3302-0732</datacite:nameIdentifier>

  
<datacite:affiliation>University of Natural Resources and Life Sciences Vienna</datacite:affiliation>

  
</datacite:creator>

  
</datacite:creators>

  
<dc:publisher>MDPI</dc:publisher>

  
<resourceType resourceTypeGeneral="literature" uri="http://purl.org/coar/resource_type/c_18cf">text</resourceType>

  
<dc:language>eng</dc:language>

  
<dc:description xml:lang="en">Fusarium is a genus that mostly consists of plant pathogenic fungi which are able to produce a broad range of toxic secondary metabolites. In this study, we focus on a type A trichothecene-producing isolate (15-39) of Fusarium sporotrichioides from Lower Austria. We assessed the secondary metabolite profile and optimized the toxin production conditions on autoclaved rice and found that in addition to large amounts of T-2 and HT-2 toxins, this strain was able to produce HT-2-glucoside. The optimal conditions for the production of T-2 toxin, HT-2 toxin, and HT-2-glucoside on autoclaved rice were incubation at 12 °C under constant light for four weeks, darkness at 30 °C for two weeks, and constant light for three weeks at 20 °C, respectively. The HT-2-glucoside was purified, and the structure elucidation by NMR revealed a mixture of two alpha-glucosides, presumably HT-2-3-O-alpha-glucoside and HT-2-4-O-alpha-glucoside. The efforts to separate the two compounds by HPLC were unsuccessful. No hydrolysis was observed with two the alpha-glucosidases or with human salivary amylase and Saccharomyces cerevisiae maltase. We propose that the two HT-2-alpha-glucosides are not formed by a glucosyltransferase as they are in plants, but by a trans-glycosylating alpha-glucosidase expressed by the fungus on the starch-containing rice medium.</dc:description>

  
<datacite:subjects>
  
<datacite:subject xml:lang="en">T-2 Toxin; Ht-2 Toxin; Mycotoxins; Glucosyltransferase; Trichothecenes; Glucosylation; Glucosides; Expansion; Family</datacite:subject>

  
</datacite:subjects>

  
<licenseCondition uri="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</licenseCondition>

  
<file mimeType="application/pdf" objectType="fulltext">https://phaidra.vetmeduni.ac.at/api/object/o:2940/download</file>

  
<datacite:alternateIdentifiers>
  
<datacite:alternateIdentifier alternateIdentifierType="DOI">10.3390/toxins16020099</datacite:alternateIdentifier>

  
</datacite:alternateIdentifiers>

  
<datacite:relatedIdentifiers>
  
<datacite:relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://phaidra.vetmeduni.ac.at/o:605</datacite:relatedIdentifier>

  
</datacite:relatedIdentifiers>

  
<dc:format>application/pdf</dc:format>

  
<dc:source>Toxins 16(2) (2024)</dc:source>

  
<citationTitle>Toxins</citationTitle>

  
<citationVolume>16</citationVolume>

  
<citationIssue>2</citationIssue>

  
<datacite:sizes>
  
<datacite:size>1.06 MB</datacite:size>

  
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<datacite:dates>
  
<datacite:date dateType="Issued">2024</datacite:date>

  
</datacite:dates>

  
</resource>