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<edm:dataProvider>University of Veterinary Medicine Vienna</edm:dataProvider>

  
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<dc:title xml:lang="en">Fusarium sporotrichioides Produces Two HT-2-α-Glucosides on Rice</dc:title>

  
<dc:description xml:lang="en">Fusarium is a genus that mostly consists of plant pathogenic fungi which are able to produce a broad range of toxic secondary metabolites. In this study, we focus on a type A trichothecene-producing isolate (15-39) of Fusarium sporotrichioides from Lower Austria. We assessed the secondary metabolite profile and optimized the toxin production conditions on autoclaved rice and found that in addition to large amounts of T-2 and HT-2 toxins, this strain was able to produce HT-2-glucoside. The optimal conditions for the production of T-2 toxin, HT-2 toxin, and HT-2-glucoside on autoclaved rice were incubation at 12 °C under constant light for four weeks, darkness at 30 °C for two weeks, and constant light for three weeks at 20 °C, respectively. The HT-2-glucoside was purified, and the structure elucidation by NMR revealed a mixture of two alpha-glucosides, presumably HT-2-3-O-alpha-glucoside and HT-2-4-O-alpha-glucoside. The efforts to separate the two compounds by HPLC were unsuccessful. No hydrolysis was observed with two the alpha-glucosidases or with human salivary amylase and Saccharomyces cerevisiae maltase. We propose that the two HT-2-alpha-glucosides are not formed by a glucosyltransferase as they are in plants, but by a trans-glycosylating alpha-glucosidase expressed by the fungus on the starch-containing rice medium.</dc:description>

  
<dc:identifier rdf:resource="https://phaidra.vetmeduni.ac.at/o:2940"></dc:identifier>

  
<dc:language>en</dc:language>

  
<edm:type>TEXT</edm:type>

  
<dc:type xml:lang="en">article</dc:type>

  
<dc:subject xml:lang="en">T-2 Toxin; Ht-2 Toxin; Mycotoxins; Glucosyltransferase; Trichothecenes; Glucosylation; Glucosides; Expansion; Family</dc:subject>

  
<dcterms:issued>2024</dcterms:issued>

  
<dc:date>2024</dc:date>

  
<dc:creator>Svoboda, Thomas (University of Natural Resources and Life Sciences Vienna)</dc:creator>

  
<dc:creator>Adam, Gerhard (University of Natural Resources and Life Sciences Vienna)</dc:creator>

  
<dc:creator>Berthiller, Franz (University of Natural Resources and Life Sciences Vienna)</dc:creator>

  
<dc:creator>Bacher, Markus (University of Natural Resources and Life Sciences Vienna / University of Veterinary Medicine Vienna)</dc:creator>

  
<dc:creator>Krska, Rudolf (University of Natural Resources and Life Sciences Vienna / Austrian Competence Centre for Feed and Food Quality, Safety and Innovation)</dc:creator>

  
<dc:creator>Labuda, Roman (University of Veterinary Medicine Vienna / Research Platform Bioactive Microbial Metabolites)</dc:creator>

  
<dc:creator>Sulyok, Michael (University of Natural Resources and Life Sciences Vienna)</dc:creator>

  
<dc:publisher>MDPI</dc:publisher>

  
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