Svoboda, Thomas (University of Natural Resources and Life Sciences Vienna) Thomas Svoboda Adam, Gerhard (University of Natural Resources and Life Sciences Vienna) Gerhard Adam Berthiller, Franz (University of Natural Resources and Life Sciences Vienna) Franz Berthiller Bacher, Markus (University of Natural Resources and Life Sciences Vienna / University of Veterinary Medicine Vienna) Markus Bacher Krska, Rudolf (University of Natural Resources and Life Sciences Vienna / Austrian Competence Centre for Feed and Food Quality, Safety and Innovation) Rudolf Krska Labuda, Roman (University of Veterinary Medicine Vienna / Research Platform Bioactive Microbial Metabolites) Roman Labuda Sulyok, Michael (University of Natural Resources and Life Sciences Vienna) Michael Sulyok MDPI 2024 Fusarium is a genus that mostly consists of plant pathogenic fungi which are able to produce a broad range of toxic secondary metabolites. In this study, we focus on a type A trichothecene-producing isolate (15-39) of Fusarium sporotrichioides from Lower Austria. We assessed the secondary metabolite profile and optimized the toxin production conditions on autoclaved rice and found that in addition to large amounts of T-2 and HT-2 toxins, this strain was able to produce HT-2-glucoside. The optimal conditions for the production of T-2 toxin, HT-2 toxin, and HT-2-glucoside on autoclaved rice were incubation at 12 °C under constant light for four weeks, darkness at 30 °C for two weeks, and constant light for three weeks at 20 °C, respectively. The HT-2-glucoside was purified, and the structure elucidation by NMR revealed a mixture of two alpha-glucosides, presumably HT-2-3-O-alpha-glucoside and HT-2-4-O-alpha-glucoside. The efforts to separate the two compounds by HPLC were unsuccessful. No hydrolysis was observed with two the alpha-glucosidases or with human salivary amylase and Saccharomyces cerevisiae maltase. We propose that the two HT-2-alpha-glucosides are not formed by a glucosyltransferase as they are in plants, but by a trans-glycosylating alpha-glucosidase expressed by the fungus on the starch-containing rice medium. PDFDocument en 2024-05-03T07:52:25.883Z T-2 Toxin; Ht-2 Toxin; Mycotoxins; Glucosyltransferase; Trichothecenes; Glucosylation; Glucosides; Expansion; Family 1108981 b application/pdf CC BY 4.0 International