phaidra.vetmeduni.ac.at o:2937 DOI 10.3390/microorganisms12020244 Detection of mcr-1-1 Positive Enteropathogenic Escherichia coli Isolates Associated with Post-Weaning Diarrhoea in an Organic Piglet-Producing Farm in Austria Postweaning diarrhoea (PWD) is a frequent multifactorial disease occurring in swine stocks worldwide. Since pathogenic Escherichia (E.) coli play a pivotal role in the pathogenesis of PWD and porcine E. coli are often resistant to different antibiotics, colistin is frequently applied to treat piglets with PWD. However, the application of colistin to livestock has been associated with the emergence of colistin resistance. This case report describes the detection of the colistin resistance gene mcr-1-1 in two E. coli isolated from piglets with PWD in an Austrian organic piglet-producing farm, which was managed by two farmers working as nurses in a hospital. Both mcr-1-positive E. coli were further analysed by Illumina short-read-sequencing, including assemblies and gene prediction. Both isolates belonged to the same clonal type and were positive for eaeH and espX5, which are both virulence genes associated with enteropathogenic E. coli (EPEC). Due to the detection of mcr-1-positive EPEC and based on the results of the antimicrobial resistance testing, the veterinarian decided to apply gentamicin for treatment instead of colistin, leading to improved clinical signs. In addition, after replacing faba beans with whey, PWD was solely observed in 2/10 weaned batches in the consecutive months. eng Resistance Gene; Weaned Pigs; Susceptibility; Identification; Salmonella; Prevalence; Belgium; Strains; Pcr 2024-05-03T07:24:59.556Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Renzhammer;René; FN:René Renzhammer X-ORCID:https://orcid.org/0000-0002-7647-8786 END:VCARD BEGIN:VCARD VERSION:3.0 N:Loncaric;Igor; FN:Igor Loncaric X-ORCID:https://orcid.org/0000-0003-1401-2961 END:VCARD BEGIN:VCARD VERSION:3.0 N:Ladinig;Andrea; FN:Andrea Ladinig X-ORCID:https://orcid.org/0000-0001-5037-7269 END:VCARD BEGIN:VCARD VERSION:3.0 N:Simetzberger;Erwin; FN:Erwin Simetzberger END:VCARD BEGIN:VCARD VERSION:3.0 N:Fuchs;Andreas; FN:Andreas Fuchs END:VCARD BEGIN:VCARD VERSION:3.0 N:Ruppitsch;Werner; FN:Werner Ruppitsch X-ORCID:https://orcid.org/0000-0001-9940-3333 END:VCARD BEGIN:VCARD VERSION:3.0 N:Schwarz;Lukas; FN:Lukas Schwarz X-ORCID:https://orcid.org/0000-0002-4716-4913 END:VCARD BEGIN:VCARD VERSION:3.0 N:Cabal Rosel;Adriana; FN:Adriana Cabal Rosel X-ORCID:https://orcid.org/0000-0001-9346-1612 END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 247806 https://phaidra.vetmeduni.ac.at/o:2937 https://phaidra.vetmeduni.ac.at/api/object/o:2937/thumbnail LOMv1.0 discipline