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<lom:identifier>
  
<lom:catalog>DOI</lom:catalog>

  
<lom:entry>
  
<lom:langstring xml:lang="x-none">10.3390/v15091942</lom:langstring>

  
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<lom:title>
  
<lom:langstring xml:lang="en">CRISPR/Cas9-Mediated Targeting of BPV-1-Transformed Primary Equine Sarcoid Fibroblasts</lom:langstring>

  
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<lom:description>
  
<lom:langstring xml:lang="en">Equine sarcoids (EqS) are fibroblast-derived skin tumors associated with bovine papillomavirus 1 and 2 (BPV-1 and -2). Based on Southern blotting, the BPV-1 genome was not found to be integrated in the host cell genome, suggesting that EqS pathogenesis does not result from insertional mutagenesis. Hence, CRISPR/Cas9 implies an interesting tool for selectively targeting BPV-1 episomes or genetically anchored suspected host factors. To address this in a proof-of-concept study, we confirmed the exclusive episomal persistence of BPV-1 in EqS using targeted locus amplification (TLA). To investigate the CRISPR/Cas9-mediated editing of BPV-1 episomes, primary equine fibroblast cultures were established and characterized. In the EqS fibroblast cultures, CRISPR-mediated targeting of the episomal E5 and E6 oncogenes as well as the BPV-1 long control region was successful and resulted in a pronounced reduction of the BPV-1 load. Moreover, the deletion of the equine Vimentin (VIM), which is highly expressed in EqS, considerably decreased the number of BPV-1 episomes. Our results suggest CRISPR/Cas9-based gene targeting may serve as a tool to help further unravel the biology of EqS pathogenesis.</lom:langstring>

  
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<lom:language>eng</lom:language>

  
<lom:keyword>
  
<lom:langstring xml:lang="en">Bovine Papillomavirus Type-1; Vimentin Intermediate-Filaments; In-Vitro; Gene-Expression; Dna; Cells; Vivo; E6; Establishment; Inactivation</lom:langstring>

  
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<lom:datetime>2024-02-26T10:45:10.720Z</lom:datetime>

  
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<lom:vcard>BEGIN:VCARD
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N:Monod;Anne;
FN:Anne Monod
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<lom:vcard>BEGIN:VCARD
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N:Hahn;Kerstin;
FN:Kerstin Hahn
X-ORCID:https://orcid.org/0000-0003-3602-4234
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<lom:vcard>BEGIN:VCARD
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N:Rottenberg;Sven;
FN:Sven Rottenberg
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<lom:vcard>BEGIN:VCARD
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N:Gerber;Vinzenz;
FN:Vinzenz Gerber
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<lom:vcard>BEGIN:VCARD
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N:Wenker;Christian;
FN:Christian Wenker
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N:Howald;Denise;
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N:Haspeslagh;Maarten;
FN:Maarten Haspeslagh
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<lom:vcard>BEGIN:VCARD
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N:Koch;Christoph;
FN:Christoph Koch
X-ORCID:https://orcid.org/0000-0002-4574-6952
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<lom:vcard>BEGIN:VCARD
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N:Jindra;Christoph;
FN:Christoph Jindra
END:VCARD</lom:vcard>

  
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