phaidra.vetmeduni.ac.at o:2283 DOI 10.1016/j.ejcb.2023.151343 3D environment controls H3K4 methylation and the mechanical response of the nucleus in acute lymphoblastic leukemia cells Acute lymphoblastic leukemia (ALL) is the most common pediatric cancer, and the infiltration of leukemic cells is critical for disease progression and relapse. Nuclear deformability plays a critical role in cancer cell invasion through confined spaces; however, the direct impact of epigenetic changes on the nuclear deformability of leukemic cells remains unclear. Here, we characterized how 3D collagen matrix conditions induced H3K4 methylation in ALL cell lines and clinical samples. We used specific shRNA and chemical inhibitors to target WDR5 (a core subunit involved in H3K4 methylation) and determined that targeting WDR5 reduced the H3K4 methylation induced by the 3D environment and the invasiveness of ALL cells in vitro and in vivo. Intriguingly, targeting WDR5 did not reduce the adhesion or the chemotactic response of leukemia cells, suggesting a different mechanism by which H3K4 methylation might govern ALL cell invasiveness. Finally, we conducted biochemical, and biophysical experiments to determine that 3D environments promoted the alteration of the chromatin, the morphology, and the mechanical behavior of the nucleus in ALL cells. Collectively, our data suggest that 3D environments control an upregulation of H3K4 methylation in ALL cells, and targeting WDR5 might serve as a promising therapeutic target against ALL invasiveness in vivo. eng High Expression; Wdr5; Infiltration; Stem; Migration; Tumorigenesis; Morphology; Shape 2023-11-08T08:54:03.097Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:González-Novo;Raquel; FN:Raquel González-Novo END:VCARD BEGIN:VCARD VERSION:3.0 N:Redondo-Muñoz;Javier; FN:Javier Redondo-Muñoz END:VCARD BEGIN:VCARD VERSION:3.0 N:Ramírez;Manuel; FN:Manuel Ramírez END:VCARD BEGIN:VCARD VERSION:3.0 N:García de Lacoba;Mario; FN:Mario García de Lacoba END:VCARD BEGIN:VCARD VERSION:3.0 N:Acitores;David; FN:David Acitores END:VCARD BEGIN:VCARD VERSION:3.0 N:de Lope-Planelles;Ana; FN:Ana de Lope-Planelles END:VCARD BEGIN:VCARD VERSION:3.0 N:Cruz Rodríguez;María Pilar; FN:María Pilar Cruz Rodríguez END:VCARD BEGIN:VCARD VERSION:3.0 N:González-Murillo;África; FN:África González-Murillo END:VCARD BEGIN:VCARD VERSION:3.0 N:Madrazo;Elena; FN:Elena Madrazo END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by-nc-nd/4.0 application/pdf 1536348 https://phaidra.vetmeduni.ac.at/o:2283 https://phaidra.vetmeduni.ac.at/api/object/o:2283/thumbnail LOMv1.0 discipline