University of Veterinary Medicine Vienna 3D environment controls H3K4 methylation and the mechanical response of the nucleus in acute lymphoblastic leukemia cells Acute lymphoblastic leukemia (ALL) is the most common pediatric cancer, and the infiltration of leukemic cells is critical for disease progression and relapse. Nuclear deformability plays a critical role in cancer cell invasion through confined spaces; however, the direct impact of epigenetic changes on the nuclear deformability of leukemic cells remains unclear. Here, we characterized how 3D collagen matrix conditions induced H3K4 methylation in ALL cell lines and clinical samples. We used specific shRNA and chemical inhibitors to target WDR5 (a core subunit involved in H3K4 methylation) and determined that targeting WDR5 reduced the H3K4 methylation induced by the 3D environment and the invasiveness of ALL cells in vitro and in vivo. Intriguingly, targeting WDR5 did not reduce the adhesion or the chemotactic response of leukemia cells, suggesting a different mechanism by which H3K4 methylation might govern ALL cell invasiveness. Finally, we conducted biochemical, and biophysical experiments to determine that 3D environments promoted the alteration of the chromatin, the morphology, and the mechanical behavior of the nucleus in ALL cells. Collectively, our data suggest that 3D environments control an upregulation of H3K4 methylation in ALL cells, and targeting WDR5 might serve as a promising therapeutic target against ALL invasiveness in vivo. en TEXT article High Expression; Wdr5; Infiltration; Stem; Migration; Tumorigenesis; Morphology; Shape 2023 2023 González-Novo, Raquel (Centro de Investigaciones Biológicas Margarita Salas) Redondo-Muñoz, Javier (Centro de Investigaciones Biológicas Margarita Salas) Ramírez, Manuel (Hospital Universitario Niño Jesús / Health Research Institute La Princesa) García de Lacoba, Mario (Centro de Investigaciones Biológicas Margarita Salas) Acitores, David (University of Veterinary Medicine Vienna) de Lope-Planelles, Ana (Centro de Investigaciones Biológicas Margarita Salas) Cruz Rodríguez, María Pilar (Centro de Investigaciones Biológicas Margarita Salas) González-Murillo, África (Hospital Universitario Niño Jesús / Health Research Institute La Princesa) Madrazo, Elena (Centro de Investigaciones Biológicas Margarita Salas) Elsevier