o:2209 Presence of Equine and Bovine Coronaviruses, Endoparasites, and Bacteria in Fecal Samples of Horses with Colic en Acute abdominal pain (colic) is one of the major equine health threats worldwide and often necessitates intensive veterinary medical care and surgical intervention. Equine coronavirus (ECoV) infections can cause colic in horses but are rarely considered as a differential diagnosis. To determine the frequency of otherwise undetected ECoV infections in horses with acute colic, fresh fecal samples of 105 horses with acute colic and 36 healthy control horses were screened for viruses belonging to the Betacoronavirus 1 species by RT-PCR as well as for gastrointestinal helminths and bacteria commonly associated with colic. Horses with colic excreted significantly fewer strongyle eggs than horses without colic. The prevalence of anaerobic, spore-forming, gram-positive bacteria (Clostridium perfringens and Clostridioides difficile) was significantly higher in the feces of horses with colic. Six horses with colic (5.7%) and one horse from the control group (2.8%) tested positive for Betacoronaviruses. Coronavirus-positive samples were sequenced to classify the virus by molecular phylogeny (N gene). Interestingly, in three out of six coronavirus-positive horses with colic, sequences closely related to bovine coronaviruses (BCoV) were found. The pathogenic potential of BCoV in horses remains unclear and warrants further investigation. Clostridium-Perfringens; Adult Horses; Infection; Pathogenicity; Prevalence; Enteritis; Sequence; Feces; Dna 1552099 10.3390/pathogens12081043 2023-10-16T11:39:38.693Z 44 yes 46 Moritz Stummer University of Veterinary Medicine Vienna 0009-0002-3744-5823 Angelika Auer University of Veterinary Medicine Vienna person 0000-0002-4790-9823 Manolis Lyrakis University of Veterinary Medicine Vienna person 0000-0003-2768-3844 Anja Joachim University of Veterinary Medicine Vienna person 0000-0003-3082-6885 Till Rümenapf University of Veterinary Medicine Vienna person 0000-0002-2951-7471 Jessika-Maximiliane V. Cavalleri University of Veterinary Medicine Vienna person 0000-0002-8575-7402 Christiane Riedel Claude Bernard University Lyon 1 person 0000-0002-4349-996X Katharina Dimmel University of Veterinary Medicine Vienna person Irina Diekmann Freie Universität Berlin person Jürgen Krücken Freie Universität Berlin person 0000-0002-2842-8100 Joachim Spergser University of Veterinary Medicine Vienna person 0000-0002-0164-0179 Barbara Hinney University of Veterinary Medicine Vienna person 0000-0001-7757-1002 Frauke Glitz Animal Clinic Würflach person Vicky Frisch University of Veterinary Medicine Vienna person application/pdf 814173 https://phaidra.vetmeduni.ac.at/o:2209 no yes 16 70 1552253 Pathogens 13 12 8 MDPI 2023