phaidra.vetmeduni.ac.at o:2209 DOI 10.3390/pathogens12081043 Presence of Equine and Bovine Coronaviruses, Endoparasites, and Bacteria in Fecal Samples of Horses with Colic Acute abdominal pain (colic) is one of the major equine health threats worldwide and often necessitates intensive veterinary medical care and surgical intervention. Equine coronavirus (ECoV) infections can cause colic in horses but are rarely considered as a differential diagnosis. To determine the frequency of otherwise undetected ECoV infections in horses with acute colic, fresh fecal samples of 105 horses with acute colic and 36 healthy control horses were screened for viruses belonging to the Betacoronavirus 1 species by RT-PCR as well as for gastrointestinal helminths and bacteria commonly associated with colic. Horses with colic excreted significantly fewer strongyle eggs than horses without colic. The prevalence of anaerobic, spore-forming, gram-positive bacteria (Clostridium perfringens and Clostridioides difficile) was significantly higher in the feces of horses with colic. Six horses with colic (5.7%) and one horse from the control group (2.8%) tested positive for Betacoronaviruses. Coronavirus-positive samples were sequenced to classify the virus by molecular phylogeny (N gene). Interestingly, in three out of six coronavirus-positive horses with colic, sequences closely related to bovine coronaviruses (BCoV) were found. The pathogenic potential of BCoV in horses remains unclear and warrants further investigation. eng Clostridium-Perfringens; Adult Horses; Infection; Pathogenicity; Prevalence; Enteritis; Sequence; Feces; Dna 2023-10-16T11:47:08.649Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Stummer;Moritz; FN:Moritz Stummer X-ORCID:https://orcid.org/0009-0002-3744-5823 END:VCARD BEGIN:VCARD VERSION:3.0 N:Auer;Angelika; FN:Angelika Auer X-ORCID:https://orcid.org/0000-0002-4790-9823 END:VCARD BEGIN:VCARD VERSION:3.0 N:Lyrakis;Manolis; FN:Manolis Lyrakis X-ORCID:https://orcid.org/0000-0003-2768-3844 END:VCARD BEGIN:VCARD VERSION:3.0 N:Joachim;Anja; FN:Anja Joachim X-ORCID:https://orcid.org/0000-0003-3082-6885 END:VCARD BEGIN:VCARD VERSION:3.0 N:Rümenapf;Till; FN:Till Rümenapf X-ORCID:https://orcid.org/0000-0002-2951-7471 END:VCARD BEGIN:VCARD VERSION:3.0 N:Cavalleri;Jessika-Maximiliane V.; FN:Jessika-Maximiliane V. Cavalleri X-ORCID:https://orcid.org/0000-0002-8575-7402 END:VCARD BEGIN:VCARD VERSION:3.0 N:Riedel;Christiane; FN:Christiane Riedel X-ORCID:https://orcid.org/0000-0002-4349-996X END:VCARD BEGIN:VCARD VERSION:3.0 N:Dimmel;Katharina; FN:Katharina Dimmel END:VCARD BEGIN:VCARD VERSION:3.0 N:Diekmann;Irina; FN:Irina Diekmann END:VCARD BEGIN:VCARD VERSION:3.0 N:Krücken;Jürgen; FN:Jürgen Krücken X-ORCID:https://orcid.org/0000-0002-2842-8100 END:VCARD BEGIN:VCARD VERSION:3.0 N:Spergser;Joachim; FN:Joachim Spergser X-ORCID:https://orcid.org/0000-0002-0164-0179 END:VCARD BEGIN:VCARD VERSION:3.0 N:Hinney;Barbara; FN:Barbara Hinney X-ORCID:https://orcid.org/0000-0001-7757-1002 END:VCARD BEGIN:VCARD VERSION:3.0 N:Glitz;Frauke; FN:Frauke Glitz END:VCARD BEGIN:VCARD VERSION:3.0 N:Frisch;Vicky; FN:Vicky Frisch END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 814173 https://phaidra.vetmeduni.ac.at/o:2209 https://phaidra.vetmeduni.ac.at/api/object/o:2209/thumbnail LOMv1.0 discipline