phaidra.vetmeduni.ac.at o:2015 DOI 10.3390/toxins14070493 Cocktails of Mycotoxins, Phytoestrogens, and Other Secondary Metabolites in Diets of Dairy Cows in Austria: Inferences from Diet Composition and Geo-Climatic Factors Dairy production is a pivotal economic sector of Austrian and European agriculture. Dietary toxins and endocrine disruptors of natural origin such as mycotoxins and phytoestrogens can affect animal health, reproduction, and productivity. This study characterized the profile of a wide spectrum of fungal, plant, and unspecific secondary metabolites, including regulated, emerging, and modified mycotoxins, phytoestrogens, and cyanogenic glucosides, in complete diets of lactating cows from 100 Austrian dairy farms. To achieve this, a validated multi-metabolite liquid chromatography/electrospray ionization-tandem mass spectrometric (LC/ESI-MS/MS) method was employed, detecting 155 of >800 tested metabolites. Additionally, the most influential dietary and geo-climatic factors related to the dietary mycotoxin contamination of Austrian dairy cattle were recognized. We evidenced that the diets of Austrian dairy cows presented ubiquitous contamination with mixtures of mycotoxins and phytoestrogens. Metabolites derived from Fusarium spp. presented the highest concentrations, were the most recurrent, and had the highest diversity among the detected fungal compounds. Zearalenone, deoxynivalenol, and fumonisin B1 were the most frequently occurring mycotoxins considered in the EU legislation, with detection frequencies >70%. Among the investigated dietary factors, inclusion of maize silage (MS) and straw in the diets was the most influential factor in contamination with Fusarium-derived and other fungal toxins and metabolites, and temperature was the most influential among the geo-climatic factors. eng Patulin-Induced Hepatotoxicity; Penicillium-Roqueforti; Fusarium-Mycotoxins; Kojic Acid; In-Vitro; 3-Nitropropionic Acid; Alternaria Mycotoxins; Multiple Mycotoxins; Ensiled Maize; Wheat Silage 2023-08-22T09:55:13.904Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Penagos-Tabares;Felipe; FN:Felipe Penagos-Tabares X-ORCID:https://orcid.org/0000-0002-4698-4741 END:VCARD BEGIN:VCARD VERSION:3.0 N:Zebeli;Qendrim; FN:Qendrim Zebeli X-ORCID:https://orcid.org/0000-0001-5188-9004 END:VCARD BEGIN:VCARD VERSION:3.0 N:Krska;Rudolf; FN:Rudolf Krska X-ORCID:https://orcid.org/0000-0001-6843-9755 END:VCARD BEGIN:VCARD VERSION:3.0 N:Sulyok;Michael; FN:Michael Sulyok X-ORCID:https://orcid.org/0000-0002-3302-0732 END:VCARD BEGIN:VCARD VERSION:3.0 N:Faas;Johannes; FN:Johannes Faas END:VCARD BEGIN:VCARD VERSION:3.0 N:Nagl;Veronika; FN:Veronika Nagl X-ORCID:https://orcid.org/0000-0002-8534-4149 END:VCARD BEGIN:VCARD VERSION:3.0 N:Kehrer;Johanna; FN:Johanna Kehrer END:VCARD BEGIN:VCARD VERSION:3.0 N:Bartl;Eva-Maria; FN:Eva-Maria Bartl END:VCARD BEGIN:VCARD VERSION:3.0 N:Schmidt;Marlene; FN:Marlene Schmidt X-ORCID:https://orcid.org/0000-0002-1426-6331 END:VCARD BEGIN:VCARD VERSION:3.0 N:Khiaosa-Ard;Ratchaneewan; FN:Ratchaneewan Khiaosa-Ard X-ORCID:https://orcid.org/0000-0003-3359-5787 END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 1442886 https://phaidra.vetmeduni.ac.at/o:2015 https://phaidra.vetmeduni.ac.at/api/object/o:2015/thumbnail LOMv1.0 discipline