o:1962 Inter-species cell detection - datasets on pulmonary hemosiderophages in equine, human and feline specimens en Pulmonary hemorrhage (P-Hem) occurs among multiple species and can have various causes. Cytology of bronchoalveolar lavage fluid (BALF) using a 5-tier scoring system of alveolar macrophages based on their hemosiderin content is considered the most sensitive diagnostic method. We introduce a novel, fully annotated multi-species P-Hem dataset, which consists of 74 cytology whole slide images (WSIs) with equine, feline and human samples. To create this high-quality and high-quantity dataset, we developed an annotation pipeline combining human expertise with deep learning and data visualisation techniques. We applied a deep learning-based object detection approach trained on 17 expertly annotated equine WSIs, to the remaining 39 equine, 12 human and 7 feline WSIs. The resulting annotations were semi-automatically screened for errors on multiple types of specialised annotation maps and finally reviewed by a trained pathologist. Our dataset contains a total of 297,383 hemosiderophages classified into five grades. It is one of the largest publicly available WSIs datasets with respect to the number of annotations, the scanned area and the number of species covered. Bronchoalveolar Lavage; Hemorrhage; Performance; Impairs 1552099 10.1038/s41597-022-01389-0 2023-08-09T13:15:37.933Z 44 yes 46 Christian Marzahl Friedrich-Alexander-Universität Erlangen-Nürnberg / EUROIMMUN Medizinische Labordiagnostika AG 0000-0001-9340-9873 Jenny Hill VetPath Laboratory Services person Jason Stayt VetPath Laboratory Services person Dorothee Bienzle University of Guelph person 0000-0002-2301-2931 Lutz Welker Lungen Clinic Grosshansdorf person Frauke Wilm Friedrich-Alexander-Universität Erlangen-Nürnberg person 0000-0002-9065-0554 Jörn Voigt EUROIMMUN Medizinische Labordiagnostika AG person Marc Aubreville Technische Hochschule Ingolstadt person Andreas Maier Friedrich-Alexander-Universität Erlangen-Nürnberg person 0000-0002-9550-5284 Robert Klopfleisch Freie Universität Berlin person 0000-0002-6308-0568 Katharina Breininger Friedrich-Alexander-Universität Erlangen-Nürnberg person Christof A. Bertram University of Veterinary Medicine Vienna / Freie Universität Berlin person 0000-0002-2402-9997 application/pdf 865152 https://phaidra.vetmeduni.ac.at/o:1962 no yes 16 70 1552253 Scientific Data 10 9 1 Nature Portfolio 2022