o:1885 Origins, phylogenetic relationships and host-parasite interactions of Troglotrematoidea since the cretaceous en In the current study, we raise the issue concerning origins and historical relationships of the trematodes from the families Troglotrematidae and Paragonimidae using phylogenetic analysis and molecular-clock method for estimating evolutionary rates. For the first time we provided 28S rRNA gene fragment (1764 bp) for the type species Troglotrema acutum - zoonotic trematodes that cause cranial lesions (troglotremiasis) in mustelid and canid mammals of the Central Europe, Iberian Peninsula, and North-West Caucasus. Molecular genetic analysis revealed that T. acutum belongs to the monophyletic family Troglotrematidae sister with the family Paragonimidae. The family Troglotrematidae includes five genera: Nanophyetus, Troglotrema, Skrjabinophyetus, Nephrotrema, and Macroorchis; and the family Paragonimidae is monotypic including the only genus Paragonimus. We recover the superfamily Troglotrematoidea for these two families. Divergence of the common ancestor of the superfamily Troglotrematoidea (common troglotrematoid ancestor) likely occurred during the Cretaceous period of the Mesozoic Era and potentially originated in the Asiatic region. The lineage of the family Troglotrematidae is much closer to the common troglotrematoid ancestor than the species of the family Paragonimidae. The radiation time of the common troglotrematoid ancestor (126 Ma, the Early Cretaceous), and formation of the families Troglotrematidae and Paragonimidae (96 Ma and 73 Ma respectively, the Late Cretaceous) corresponds to the time of settling in East Asia by many species of mammaliaforms (about 130-70 Ma) Trematoda Troglotrematidae; Acutum Digenea; Ribosomal-Rna; Platyhelminthes; Divergence; Utility; Genes; Meles 1552099 10.1016/j.meegid.2022.105274 2023-07-25T08:11:37.234Z 44 yes 46 Konstantin S. Vainutis Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing / Far Eastern Federal University Anastasia N. Voronova Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing / Far Eastern Federal University person Georg G. Duscher University of Veterinary Medicine Vienna / AGES-Austrian Agency for Health & Food Safety person 0000-0002-3486-5470 Egor M. Shchelkanov Moscow Region State University person Mikhail Yu Shchelkanov Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing / Far Eastern Federal University person application/pdf 2088400 https://phaidra.vetmeduni.ac.at/o:1885 no yes 18 70 1552253 Infection, Genetics and Evolution 8 101 Elsevier 2022