o:1748 Comparing head muscles among Drusinae clades (Insecta: Trichoptera) reveals high congruence despite strong contrasts in head shape en The subfamily Drusinae (Limnephilidae, Trichoptera) comprises a range of species exhibiting differently shaped head capsules in their larval stages. These correspond to evolutionary lineages pursuing different larval feeding ecologies, each of which uses a different hydraulic niche: scraping grazers and omnivorous shredders sharing rounded head capsules and filtering carnivores with indented and corrugated head capsules. In this study, we assess whether changes in head capsule morphology are reflected by changes in internal anatomy of Drusinae heads. To this end, internal and external head morphology was visualized using µCT methods and histological sections in three Drusinae species-Drusus franzi, D. discolor and D. bosnicus-representing the three evolutionary lineages. Our results indicate that Drusinae head musculature is highly conserved across the evolutionary lineages with only minute changes between taxa. Conversely, the tentorium is reduced in D. discolor, the species with the most aberrant head capsule investigated here. Integrating previous research on Drusinae head anatomy, we propose a fundamental Drusinae blueprint comprising 29 cephalic muscles and discuss significance of larval head capsule corrugation in Trichoptera. Particulate Organic-Matter; Feeding Ecology; Hydraulic Habitat; Limnephilidae; Evolution; Velocity 1552099 10.1038/s41598-022-04790-2 2023-06-20T13:01:18.642Z 44 yes 46 Carina Zittra University of Vienna 0000-0002-8963-6421 Johann Waringer University of Vienna person Hendrik C. Kuhlmann Technical University of Vienna person Ariane Vieira Technical University of Vienna person Jan Martini WasserCluster Lunz - Biologische Station GmbH / University of Innsbruck person Stephan Handschuh University of Veterinary Medicine Vienna person 0000-0002-2140-7892 Simon Vitecek WasserCluster Lunz - Biologische Station GmbH / University of Natural Resources and Life Sciences person Thomas Schwaha University of Vienna person application/pdf 3709266 https://phaidra.vetmeduni.ac.at/o:1748 no yes 16 70 1552253 Scientific Reports 12 12 1 Nature Publishing Group 2022