https://phaidra.vetmeduni.ac.at/o:1652 Identification of in vivo induced antigens of the malacosporean parasite Tetracapsuloides bryosalmonae (Cnidaria) using in vivo induced antigen technology Kumar, Gokhlesh Gokhlesh Kumar 0000-0002-1958-2579 University of Veterinary Medicine Vienna El-Matbouli, Mansour Mansour El-Matbouli 0000-0001-8148-0218 University of Veterinary Medicine Vienna Bartholomew, Jerri L. Jerri L. Bartholomew Nilsen, Frank Frank Nilsen Shivam, Saloni Saloni Shivam University of Veterinary Medicine Vienna Sudhagar, Arun Arun Sudhagar University of Veterinary Medicine Vienna Frontiers Media Sa text eng Tetracapsuloides bryosalmonae is a malacosporean endoparasite that causes proliferative kidney disease (PKD) in wild and farmed salmonids in Europe and North America. The life cycle of T. bryosalmonae completes between invertebrate bryozoan and vertebrate fish hosts. Inside the fish, virulence factors of T. bryosalmonae are induced during infection or interactions with host cells. T. bryosalmonae genes expressed in vivo are likely to be important in fish pathogenesis. Herein, we identify in vivo induced antigens of T. bryosalmonae during infection in brown trout (Salmo trutta) using in vivo induced antigen technology (IVIAT). Brown trout were exposed to the spores of T. bryosalmonae and were sampled at different time points. The pooled sera were first pre-adsorbed with antigens to remove false positive results. Subsequently, adsorbed sera were used to screen a T. bryosalmonae cDNA phage expression library. Immunoscreening analysis revealed 136 immunogenic T. bryosalmonae proteins induced in brown trout during parasite development. They are involved in signal transduction, transport, metabolism, ion-protein binding, protein folding, and also include hypothetical proteins, of so far unknown functions. The identified in vivo induced antigens will be useful in the understanding of T. bryosalmonae pathogenesis during infection in susceptible hosts. Some of the antigens found may have significant implications for the discovery of candidate molecules for the development of potential therapies and preventive measures against T. bryosalmonae in salmonids. myxozoa proliferative kidney disease (PKD) etracapsuloides bryosalmonae antigen in vivo induced antigens (IVIAT) host-parasite interaction salmonids http://creativecommons.org/licenses/by/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:1652/download 10.3389/fcimb.2022.1032347 https://phaidra.vetmeduni.ac.at/o:605 application/pdf Frontiers in Cellular and Infection Microbiology 12 (2022) Frontiers in Cellular and Infection Microbiology 12 5.68 MB 2022