o:1573 One Health Surveillance Highlights Circulation of Viruses with Zoonotic Potential in Bats, Pigs, and Humans in Viet Nam en A One Health cross-sectoral surveillance approach was implemented to screen biological samples from bats, pigs, and humans at high-risk interfaces for zoonotic viral spillover for five viral families with zoonotic potential in Viet Nam. Over 1600 animal and human samples from bat guano harvesting sites, natural bat roosts, and pig farming operations were tested for coronaviruses (CoVs), paramyxoviruses, influenza viruses, filoviruses and flaviviruses using consensus PCR assays. Human samples were also tested using immunoassays to detect antibodies against eight virus groups. Significant viral diversity, including CoVs closely related to ancestors of pig pathogens, was detected in bats roosting at the human-animal interfaces, illustrating the high risk for CoV spillover from bats to pigs in Viet Nam, where pig density is very high. Season and reproductive period were significantly associated with the detection of bat CoVs, with site-specific effects. Phylogeographic analysis indicated localized viral transmission among pig farms. Our limited human sampling did not detect any known zoonotic bat viruses in human communities living close to the bat cave and harvesting bat guano, but our serological assays showed possible previous exposure to Marburg virus-like (Filoviridae), Crimean-Congo hemorrhagic fever virus-like (Bunyaviridae) viruses and flaviviruses. Targeted and coordinated One Health surveillance helped uncover this viral pathogen emergence hotspot. Encephalitis-Virus; Usutu-Virus; Nipah Virus; Coronavirus; Identification; Paramyxovirus; Chiroptera; History; Origin; Growth 1552099 10.3390/v15030790 2023-04-25T11:10:22.830Z 44 yes 46 Amanda E. Fine Wildlife Conservation Society person 0000-0003-1163-5514 Sarah H Olson Wildlife Conservation Society person 0000-0002-8484-9006 Chris Walzer University of Veterinary Medicine, Vienna person 0000-0002-0437-5147 Jonna A K Mazet University of California person Christine K Johnson University of California person Pawin Padungtod Food and Agriculture Organization of the United Nations person 0000-0001-8861-0919 Ken Inui Food and Agriculture Organization of the United Nations person Keersten Ricks U.S. Army Medical Research Institute of Infectious Diseases person Randal Schoepp U.S. Army Medical Research Institute of Infectious Diseases person Nguyen Duc Thinh Ministry of Health person Vu Trong Duoc Ministry of Health person Vu Sinh Nam Ministry of Health person Nguyen Tung Ministry of Agricultural and Rural Development of Viet Nam person Le Tin Vinh Quang Regional Animal Health Office No. 6 person Nguyen Thanh Phuong Regional Animal Health Office No. 6 person Pham Thanh long Ministry of Agricultural and Rural Development of Viet Nam person 0000-0001-7093-7032 Nguyen Van Long Ministry of Agricultural and Rural Development of Viet Nam person Hoang Bich Thuy Wildlife Conservation Society person Pham Thi Bich Ngoc Wildlife Conservation Society person Nguyen Van Long Wildlife Conservation Society person Alice Latinne Wildlife Conservation Society person 0000-0003-1249-2025 Nguyen Thi Thanh Nga Wildlife Conservation Society person 46 Predict Consortium institution application/pdf 3345134 https://phaidra.vetmeduni.ac.at/o:1573 no yes 16 70 1552253 Viruses 24 15 3 MDPI 2023