https://phaidra.vetmeduni.ac.at/o:1573 One Health Surveillance Highlights Circulation of Viruses with Zoonotic Potential in Bats, Pigs, and Humans in Viet Nam Fine, Amanda E. Amanda E. Fine 0000-0003-1163-5514 Wildlife Conservation Society Olson, Sarah H Sarah H Olson 0000-0002-8484-9006 Wildlife Conservation Society Walzer, Chris Chris Walzer 0000-0002-0437-5147 University of Veterinary Medicine, Vienna Mazet, Jonna A K Jonna A K Mazet University of California Johnson, Christine K Christine K Johnson University of California Padungtod, Pawin Pawin Padungtod 0000-0001-8861-0919 Food and Agriculture Organization of the United Nations Inui, Ken Ken Inui Food and Agriculture Organization of the United Nations Ricks, Keersten Keersten Ricks U.S. Army Medical Research Institute of Infectious Diseases Schoepp, Randal Randal Schoepp U.S. Army Medical Research Institute of Infectious Diseases Thinh, Nguyen Duc Nguyen Duc Thinh Ministry of Health Duoc, Vu Trong Vu Trong Duoc Ministry of Health Nam, Vu Sinh Vu Sinh Nam Ministry of Health Tung, Nguyen Nguyen Tung Ministry of Agricultural and Rural Development of Viet Nam Quang, Le Tin Vinh Le Tin Vinh Quang Regional Animal Health Office No. 6 Phuong, Nguyen Thanh Nguyen Thanh Phuong Regional Animal Health Office No. 6 long, Pham Thanh Pham Thanh long 0000-0001-7093-7032 Ministry of Agricultural and Rural Development of Viet Nam Long, Nguyen Van Nguyen Van Long Ministry of Agricultural and Rural Development of Viet Nam Thuy, Hoang Bich Hoang Bich Thuy Wildlife Conservation Society Ngoc, Pham Thi Bich Pham Thi Bich Ngoc Wildlife Conservation Society Long, Nguyen Van Nguyen Van Long Wildlife Conservation Society Latinne, Alice Alice Latinne 0000-0003-1249-2025 Wildlife Conservation Society Nga, Nguyen Thi Thanh Nguyen Thi Thanh Nga Wildlife Conservation Society Predict Consortium MDPI text eng A One Health cross-sectoral surveillance approach was implemented to screen biological samples from bats, pigs, and humans at high-risk interfaces for zoonotic viral spillover for five viral families with zoonotic potential in Viet Nam. Over 1600 animal and human samples from bat guano harvesting sites, natural bat roosts, and pig farming operations were tested for coronaviruses (CoVs), paramyxoviruses, influenza viruses, filoviruses and flaviviruses using consensus PCR assays. Human samples were also tested using immunoassays to detect antibodies against eight virus groups. Significant viral diversity, including CoVs closely related to ancestors of pig pathogens, was detected in bats roosting at the human-animal interfaces, illustrating the high risk for CoV spillover from bats to pigs in Viet Nam, where pig density is very high. Season and reproductive period were significantly associated with the detection of bat CoVs, with site-specific effects. Phylogeographic analysis indicated localized viral transmission among pig farms. Our limited human sampling did not detect any known zoonotic bat viruses in human communities living close to the bat cave and harvesting bat guano, but our serological assays showed possible previous exposure to Marburg virus-like (Filoviridae), Crimean-Congo hemorrhagic fever virus-like (Bunyaviridae) viruses and flaviviruses. Targeted and coordinated One Health surveillance helped uncover this viral pathogen emergence hotspot. Encephalitis-Virus; Usutu-Virus; Nipah Virus; Coronavirus; Identification; Paramyxovirus; Chiroptera; History; Origin; Growth http://creativecommons.org/licenses/by/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:1573/download 10.3390/v15030790 https://phaidra.vetmeduni.ac.at/o:605 application/pdf Viruses 15(3) (2023) Viruses 15 3 3.19 MB 2023