Fine, Amanda E. (Wildlife Conservation Society) Amanda E. Fine Olson, Sarah H (Wildlife Conservation Society) Sarah H Olson Duoc, Vu Trong (Ministry of Health) Vu Trong Duoc Nam, Vu Sinh (Ministry of Health) Vu Sinh Nam Tung, Nguyen (Ministry of Agricultural and Rural Development of Viet Nam) Nguyen Tung Quang, Le Tin Vinh (Regional Animal Health Office No. 6) Le Tin Vinh Quang Phuong, Nguyen Thanh (Regional Animal Health Office No. 6) Nguyen Thanh Phuong long, Pham Thanh (Ministry of Agricultural and Rural Development of Viet Nam) Pham Thanh long Long, Nguyen Van (Ministry of Agricultural and Rural Development of Viet Nam) Nguyen Van Long Thuy, Hoang Bich (Wildlife Conservation Society) Hoang Bich Thuy Ngoc, Pham Thi Bich (Wildlife Conservation Society) Pham Thi Bich Ngoc Long, Nguyen Van (Wildlife Conservation Society) Nguyen Van Long Walzer, Chris (University of Veterinary Medicine, Vienna) Chris Walzer Latinne, Alice (Wildlife Conservation Society) Alice Latinne Nga, Nguyen Thi Thanh (Wildlife Conservation Society) Nguyen Thi Thanh Nga Mazet, Jonna A K (University of California) Jonna A K Mazet Johnson, Christine K (University of California) Christine K Johnson Padungtod, Pawin (Food and Agriculture Organization of the United Nations) Pawin Padungtod Inui, Ken (Food and Agriculture Organization of the United Nations) Ken Inui Ricks, Keersten (U.S. Army Medical Research Institute of Infectious Diseases) Keersten Ricks Schoepp, Randal (U.S. Army Medical Research Institute of Infectious Diseases) Randal Schoepp Thinh, Nguyen Duc (Ministry of Health) Nguyen Duc Thinh Predict Consortium MDPI 2023 A One Health cross-sectoral surveillance approach was implemented to screen biological samples from bats, pigs, and humans at high-risk interfaces for zoonotic viral spillover for five viral families with zoonotic potential in Viet Nam. Over 1600 animal and human samples from bat guano harvesting sites, natural bat roosts, and pig farming operations were tested for coronaviruses (CoVs), paramyxoviruses, influenza viruses, filoviruses and flaviviruses using consensus PCR assays. Human samples were also tested using immunoassays to detect antibodies against eight virus groups. Significant viral diversity, including CoVs closely related to ancestors of pig pathogens, was detected in bats roosting at the human-animal interfaces, illustrating the high risk for CoV spillover from bats to pigs in Viet Nam, where pig density is very high. Season and reproductive period were significantly associated with the detection of bat CoVs, with site-specific effects. Phylogeographic analysis indicated localized viral transmission among pig farms. Our limited human sampling did not detect any known zoonotic bat viruses in human communities living close to the bat cave and harvesting bat guano, but our serological assays showed possible previous exposure to Marburg virus-like (Filoviridae), Crimean-Congo hemorrhagic fever virus-like (Bunyaviridae) viruses and flaviviruses. Targeted and coordinated One Health surveillance helped uncover this viral pathogen emergence hotspot. PDFDocument en 2023-04-25T11:10:22.830Z Encephalitis-Virus; Usutu-Virus; Nipah Virus; Coronavirus; Identification; Paramyxovirus; Chiroptera; History; Origin; Growth 3345134 b application/pdf CC BY 4.0 International