https://phaidra.vetmeduni.ac.at/o:1515 From Gut to Blood: Spatial and Temporal Pathobiome Dynamics during Acute Abdominal Murine Sepsis Hartwig, Christina Christina Hartwig 0000-0001-9379-1435 Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB / University of Stuttgart Sohn, Kai Kai Sohn Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB Osuchowski, Marcin Marcin Osuchowski 0000-0002-1321-9883 Ludwig Boltzmann Institute for Traumatology the Research Centre in Cooperation with AUVA Ehling-Schulz, Monika Monika Ehling-Schulz 0000-0001-7384-0594 University of Veterinary Medicine, Vienna Schmitt, Theresa Theresa Schmitt Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB Maneth, Madeline Madeline Maneth Innovation Field In-Vitro Diagnostics, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB Vainshtein, Yevhen Yevhen Vainshtein 0000-0001-7944-8892 Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB Drechsler, Susanne Susanne Drechsler Ludwig Boltzmann Institute for Traumatology the Research Centre in Cooperation with AUVA MDPI text eng Abdominal sepsis triggers the transition of microorganisms from the gut to the peritoneum and bloodstream. Unfortunately, there is a limitation of methods and biomarkers to reliably study the emergence of pathobiomes and to monitor their respective dynamics. Three-month-old CD-1 female mice underwent cecal ligation and puncture (CLP) to induce abdominal sepsis. Serial and terminal endpoint specimens were collected for fecal, peritoneal lavage, and blood samples within 72 h. Microbial species compositions were determined by NGS of (cell-free) DNA and confirmed by microbiological cultivation. As a result, CLP induced rapid and early changes of gut microbial communities, with a transition of pathogenic species into the peritoneum and blood detected at 24 h post-CLP. NGS was able to identify pathogenic species in a time course-dependent manner in individual mice using cfDNA from as few as 30 microliters of blood. Absolute levels of cfDNA from pathogens changed rapidly during acute sepsis, demonstrating its short half-life. Pathogenic species and genera in CLP mice significantly overlapped with pathobiomes from septic patients. The study demonstrated that pathobiomes serve as reservoirs following CLP for the transition of pathogens into the bloodstream. Due to its short half-life, cfDNA can serve as a precise biomarker for pathogen identification in blood. Septic Shock; Microbiota; Model; Mice; Dna http://creativecommons.org/licenses/by/4.0/ https://phaidra.vetmeduni.ac.at/api/object/o:1515/download 10.3390/microorganisms11030627 https://phaidra.vetmeduni.ac.at/o:605 application/pdf Microorganisms 11(3) (2023) Microorganisms 11 3 3.35 MB 2023