phaidra.vetmeduni.ac.at o:1379 DOI 10.3390/jof9020220 Detection of Pneumocystis and Morphological Description of Fungal Distribution and Severity of Infection in Thirty-Six Mammal Species Pneumocystis spp. are thought to adapt to the lungs of potentially all mammals. However, the full host range, fungal burden and severity of infection are unknown for many species. In this study, lung tissue samples originating from 845 animals of 31 different families of eight mammal orders were screened by in situ hybridization (ISH) using a universal 18S rRNA probe for Pneumocystis, followed by hematoxylin and eosin (H&E) staining for determining histopathological lesions. A total of 216 (26%) samples were positive for Pneumocystis spp., encompassing 36 of 98 investigated mammal species, with 17 of them being described for the first time for the presence of Pneumocystis spp. The prevalence of Pneumocystis spp. as assessed by ISH varied greatly among different mammal species while the organism load was overall low, suggesting a status of colonization or subclinical infection. Severe Pneumocystis pneumonia seemed to be very rare. For most of the Pneumocystis-positive samples, comparative microscopic examination of H&E- and ISH-stained serial sections revealed an association of the fungus with minor lesions, consistent with an interstitial pneumonia. Colonization or subclinical infection of Pneumocystis in the lung might be important in many mammal species because the animals may serve as a reservoir. eng Glires Primates Perissodactyla Eulipotyphla Chiroptera Carnivora Artiodactyla Lung Histopathology In Situ Hybridization Pneumocystis Species 2023-03-01T10:15:06.015Z LOMv1.0 Author BEGIN:VCARD VERSION:3.0 N:Weissenbacher-Lang;Christiane; FN:Christiane Weissenbacher-Lang X-ORCID:https://orcid.org/0000-0002-1527-7593 END:VCARD BEGIN:VCARD VERSION:3.0 N:Blasi;Barbara; FN:Barbara Blasi X-ORCID:https://orcid.org/0000-0002-3776-6363 END:VCARD BEGIN:VCARD VERSION:3.0 N:Bauer;Patricia; FN:Patricia Bauer END:VCARD BEGIN:VCARD VERSION:3.0 N:Binanti;Diana; FN:Diana Binanti END:VCARD BEGIN:VCARD VERSION:3.0 N:Bittermann;Karin; FN:Karin Bittermann END:VCARD BEGIN:VCARD VERSION:3.0 N:Ergin;Lara; FN:Lara Ergin END:VCARD BEGIN:VCARD VERSION:3.0 N:Högler;Carmen; FN:Carmen Högler END:VCARD BEGIN:VCARD VERSION:3.0 N:Högler;Tanja; FN:Tanja Högler END:VCARD BEGIN:VCARD VERSION:3.0 N:Klier;Magdalena; FN:Magdalena Klier END:VCARD BEGIN:VCARD VERSION:3.0 N:Matt;Julia; FN:Julia Matt END:VCARD BEGIN:VCARD VERSION:3.0 N:Nedorost;Nora; FN:Nora Nedorost END:VCARD BEGIN:VCARD VERSION:3.0 N:Silvestri;Serenella; FN:Serenella Silvestri X-ORCID:https://orcid.org/0000-0002-2366-1210 END:VCARD BEGIN:VCARD VERSION:3.0 N:Stixenberger;Daniela; FN:Daniela Stixenberger END:VCARD BEGIN:VCARD VERSION:3.0 N:Ma;Liang; FN:Liang Ma X-ORCID:https://orcid.org/0000-0002-6078-5730 END:VCARD BEGIN:VCARD VERSION:3.0 N:Cissé;Ousmane H.; FN:Ousmane H. Cissé X-ORCID:https://orcid.org/0000-0002-2990-2185 END:VCARD BEGIN:VCARD VERSION:3.0 N:Kovacs;Joseph A.; FN:Joseph A. Kovacs X-ORCID:https://orcid.org/0000-0002-5191-9880 END:VCARD BEGIN:VCARD VERSION:3.0 N:Desvars-Larrive;Amélie; FN:Amélie Desvars-Larrive X-ORCID:https://orcid.org/0000-0001-7671-696X END:VCARD BEGIN:VCARD VERSION:3.0 N:Posautz;Annika; FN:Annika Posautz X-ORCID:https://orcid.org/0000-0003-4924-6733 END:VCARD BEGIN:VCARD VERSION:3.0 N:Weissenböck;Herbert; FN:Herbert Weissenböck X-ORCID:https://orcid.org/0000-0001-8197-6379 END:VCARD https://w3id.org/kim/hcrt/scheme https://w3id.org/kim/hcrt/text Textdokument Text LOMv1.0 yes https://creativecommons.org/licenses/by/4.0 application/pdf 6686199 https://phaidra.vetmeduni.ac.at/o:1379 https://phaidra.vetmeduni.ac.at/api/object/o:1379/thumbnail LOMv1.0 discipline